Physical mapping and identification of interspersed homologous sequences in the Trichoplusia ni granulosis virus genome

The Journal of General Virology
Y HashimotoT Matsumoto

Abstract

A restriction fragment library representing 89.3% of the genome of Trichoplusia ni granulosis virus (TnGV) was constructed. The library consisted of 13 of the 16 BamHI fragments, 18 of the 22 EcoRI fragments, and 6 of the 27 PstI fragments. By restriction endonuclease and Southern blot analysis of cloned or genomic viral DNA fragments, a complete physical map of TnGV was constructed for BamHI, EcoRI, PstI and XhoI. Three interspersed homologous regions (ihs1-ihs3) were identified from hybridization experiments and sequenced. Each TnGV ihs has an approximate size of 400 bp and shows homology to the other two. The orientation of ihs2 is inverted relative to ihs1 and ihs3. TnGV ihs regions do not have repetitive motifs or palindromic sequences, in contrast to homologous regions (hrs) of nuclear polyhedrosis viruses (NPVs). The genomic locations of TnGV ihs1-ihs3, represented in percentage map units, were very similar to those of ihs sequences previously reported in Bombyx mori NPV, suggesting that the ihs may be a novel type of cis-acting element common among baculoviruses. Additionally, an inverted repeat sequence, having overlapping, multiple inverted repeats of 400 bp, was identified to the left of ihs3 on the linearized genome...Continue Reading

Citations

Jul 27, 2001·Applied and Environmental Microbiology·A Sciocco-CapV Romanowski
Jun 14, 2000·Journal of Invertebrate Pathology·C M FinnertyR R Granados
Oct 14, 1998·Japanese Journal of Clinical Oncology·Y M LiuS H Yen
Mar 18, 2008·The Journal of General Virology·Shigeyuki Mukawa, Chie Goto
Sep 30, 1999·Virology·T HayakawaS Maeda
Sep 22, 2000·Virology·Y HashimotoT Matsumoto

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