Physical mapping of 18S rDNA cistron in species of the Omophoita genus (Coleoptera, Alticinae) using fluorescent in situ hybridization

Micron : the International Research and Review Journal for Microscopy
Mara Cristina AlmeidaMarcelo Ricardo Vicari

Abstract

Alticinae has the greatest amount of biodiversity among the Chrysomelidae, with 40,000 described species, only 290 of which have been analyzed cytogenetically. The majority of studies refer to conventional staining and few species have been analyzed or have responded to differential staining methods. The aim of the present study was to describe an 18S rDNA probe for Alticinae and the location of this cluster in species of the Omophoita genus. The fragment of approximately 750bp obtained through a PCR (Polymerase Chain Reaction) amplification reaction with specific oligonucleotides to 18S rDNA was cloned and denominated pTZ_Ooct_18Sp and then submitted to automatic sequencing. The alignment of the sequences obtained through the sequencing of the clones generated a consensus sequence of 722bp for Omophoita octoguttata with 98% homology with other species of Alticinae. The analysis of mitotic cells of O. octoguttata and Omophoita magniguttis submitted to fluorescent in situ hybridization (FISH) with the 18S rDNA probe revealed that the ribosomal genes are located in 6th pair. O. magniguttis also has a second labeled pair. Omophoita personata exhibited nucleolar organizer regions associated to one autosome pair. The analysis of mei...Continue Reading

References

Nov 25, 1988·Nucleic Acids Research·F Corpet
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ W Gray
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Sep 1, 1993·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·C JuanE Petitpierre
Aug 26, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·R VitturiM Zunino
Jul 13, 2000·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Z ZhangW Miller
Aug 4, 2004·Micron : the International Research and Review Journal for Microscopy·Mariastella ColombaMario Zunino
Sep 6, 2005·Micron : the International Research and Review Journal for Microscopy·Mariastella ColombaMario Zunino
Nov 23, 2006·Micron : the International Research and Review Journal for Microscopy·Marielle Cristina SchneiderDoralice Maria Cella
Oct 1, 2009·Genetics and Molecular Biology·Maressa FerreiraOrlando Moreira-Filho

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Citations

Apr 17, 2013·Micron : the International Research and Review Journal for Microscopy· Ravindra KumarNaresh S Nagpure
Sep 13, 2018·Genetica·Crislaine Vanessa UbinskiMarielle Cristina Schneider
Nov 20, 2020·Genetics and Molecular Biology·José Paulo da Costa Pinto NetoCarlos Henrique Schneider

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