Physical mapping of Mycobacterium bovis BCG pasteur reveals differences from the genome map of Mycobacterium tuberculosis H37Rv and from M. bovis

Microbiology
W J PhilippS T Cole

Abstract

A Dral restriction map of the approximately 4.35 Mb circular chromosome of the vaccine strain Mycobacterium bovis BCG Pasteur was constructed by linking all 21 Dral fragments, ranging in size from 6 to 820 kb, using specific clones that spanned the Dral recognition sites as hybridization probes. The positions of 20 known genes were also established. Comparison of the resultant genome map with that of the virulent tubercle bacillus Mycobacterium tuberculosis H37Rv revealed extensive global conservation of the genomes of these two members of the M. tuberculosis complex. Possible sites of evolutionary rearrangements were localized on the chromosome of M. bovis BCG Pasteur by comparing the Asnl restriction profile with that of M. tuberculosis H37Rv. When selected cosmids from the corresponding areas of the genome of M. tuberculosis H37Rv were used as hybridization probes to examine different BCG strains, wild-type M. bovis and M. tuberculosis H37Rv, a number of deletions up to 10 kb in size, insertions and other polymorphisms were detected. In addition to the known deletions covering the genes for the protein antigens ESAT-6 and mpt64, other genetic loci exhibiting polymorphisms or rearrangements were detected in M. bovis BCG Pasteur.

Citations

Apr 3, 2001·Journal of Molecular Spectroscopy·J.-P. BouanichA. Picard-Bersellini
Jul 18, 2001·Vaccine·N Ohara, T Yamada
Jun 24, 2000·Research in Microbiology·R BroschS T Cole
Sep 13, 2001·Trends in Microbiology·R BroschS T Cole
Oct 3, 2001·Microbes and Infection·P R Jungblut
Oct 25, 2000·International Journal of Medical Microbiology : IJMM·R BroschS T Cole
Jun 30, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H M VordermeierR G Hewinson
Jun 30, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A WilliamsR G Hewinson
Jan 1, 2003·The Journal of Infectious Diseases·Kaeryn N LewisDavid R Sherman
Sep 10, 2002·Journal of Bacteriology·R D FleischmannC M Fraser
Feb 3, 1999·Annual Review of Genetics·S Casjens
Jan 17, 2009·BMC Genomics·Chia-wei WuAdel M Talaat
Sep 17, 2008·BMC Genomics·Andrea S LeungJun Liu
Oct 28, 2011·BMC Infectious Diseases·Janeth A Almaguer-ChávezLucio Vera-Cabrera
Jun 19, 2001·Biological Chemistry·H MollenkopfS H Kaufmann
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Roland BroschStewart T Cole
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Monica L BurtsDominique M Missiakas
May 22, 2008·Molekuliarnaia genetika, mikrobiologiia i virusologiia·V V DemkinA M Dauletbakova
Jul 3, 2003·Médecine sciences : M/S·Dominique Labie
May 20, 1998·Electrophoresis·W J PhilippS T Cole
May 10, 2001·Scandinavian Journal of Infectious Diseases·M A Behr
Apr 16, 2003·Critical Reviews in Biotechnology·Ashish ParadkarDiane Stassi
Sep 16, 2009·Scandinavian Journal of Immunology·O ParkashM Pai
May 16, 2006·Microbes and Infection·Ren Zhang, Chun-Ting Zhang
Apr 20, 2005·Clinics in Chest Medicine·Daniel Brodie, Neil W Schluger
Jul 4, 2012·Vaccine·Katarzyna Krysztopa-GrzybowskaAnna Lutyńska
Aug 17, 2006·FEMS Microbiology Reviews·Mickaël Desvaux, Michel Hébraud
Sep 29, 2004·FEMS Microbiology Reviews·Volker GürtlerRobert Seviour
Jul 21, 2001·Tuberculosis·S V GordonR G Hewinson
Jan 13, 2009·Journal of Occupational Medicine and Toxicology·Anja SchablonAlbert Nienhaus
Jun 8, 2012·PloS One·Stephen H-F MacdonaldJoseph Keane

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.