Physical mapping of the Tec and Gabrb1 loci reveals that the Wsh mutation on mouse chromosome 5 is associated with an inversion

Human Molecular Genetics
D L NagleM Bućan

Abstract

In the mouse, mutations in the c-Kit proto-oncogene, a member of the receptor tyrosine kinase (RTK) gene family, have pleiotropic effects on hematopoiesis, pigmentation and fertility (dominant spotting, W). However, in the Wsh allele the defect is confined to abnormal pigmentation caused by the disruption of 5' regulatory sequences of Kit leaving an intact structural gene. In this report, the previously published physical map around the Pdgfra-Kit-Flk1 RTK loci is extended by mapping the loci encoding the GABAA (gamma-aminobutyric acid) receptor subunit beta 1, Gabrb1 and a cytoplasmic kinase (Tec) 3 Mb proximal to Kit. PFGE analysis of the wild-type (C57BL/6J) chromosome demonstrates the following gene order: cen-Gabrb1-Tec-Pdgfra-Kit, whereas the analysis of Wsh/Wsh DNA is consistent with the order: cen-Gabrb1-Pdgfra-Tec-Kit. This altered physical map can be explained by an inversion on the Wsh chromosome located proximally to the Kit locus and spanning the 2.8 Mb Pdgfra-Tec chromosomal segment. This high resolution physical mapping study identifies large DNA fragments that span the two inversion breakpoints and potentially carry Kit upstream regulatory elements involved in the control of Kit expression during embryonic devel...Continue Reading

Citations

Sep 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E BentleyS M Darling
Jan 19, 2013·Cell Death & Disease·K WoidackiA C Zenclussen
Apr 8, 2011·American Journal of Respiratory and Critical Care Medicine·James Y LindseyJohn W Hollingsworth
Feb 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Peter A NigrovicDavid M Lee
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·R B HoughM Bućan
Oct 5, 2011·The Journal of Experimental Medicine·Adeline PorcherieSalaheddine Mécheri
Jul 19, 2006·Molecular and Cellular Biology·Georgina BerrozpePeter Besmer
Nov 7, 2012·Trends in Immunology·Laurent L ReberStephen J Galli
Oct 13, 2009·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Satoshi ItohMichael P Fischbein
Aug 20, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M CyphertB H Koller
Dec 22, 2009·Journal of Cellular Biochemistry·Hanna M HeikkiläKen A Lindstedt
Aug 30, 2005·The American Journal of Pathology·Michele A GrimbaldestonStephen J Galli
Oct 24, 2009·Developmental Biology·Jennifer N Lilla, Zena Werb
Aug 17, 2016·Scientific Reports·Sutada Lotinun, Nateetip Krishnamra
Jan 13, 2018·International Journal of Molecular Sciences·Scott P Levick, Alexander Widiapradja
Feb 7, 2008·Cytogenetic and Genome Research·S A BrooksE Bailey
Oct 10, 2018·International Immunology·Akihiko MurataShin-Ichi Hayashi
Mar 1, 2019·Journal of Leukocyte Biology·Elena JachettiBarbara Frossi
Oct 8, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael R ConventeEileen M Shore
Jan 23, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M KlüppelA Bernstein
Mar 18, 2015·Experimental Dermatology·Nina A SchweintzgerPeter Wolf
Mar 30, 2018·Frontiers in Immunology·Melissa A Brown
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·D James HaddonKelly M McNagny
Dec 21, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Joseph D HernandezStephen J Galli
May 8, 1998·Methods : a Companion to Methods in Enzymology·L Flaherty
Oct 20, 2021·Journal of Neurochemistry·Pablo Eliasib Martínez-GoparFrancisca Pérez-Severiano

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