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Physical parameters affecting the rate and completion of RNA driven hybridization of DNA: new measurements relevant to quantitation based on kinetics

Nucleic Acids Research

Dec 20, 1982

Jeffrey Van Ness, W E Hahn

PMID: 6186985

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Abstract

Differences in the RNA-driven hybridization kinetics of genomic DNA and cDNA probes led us to examine physical parameters affecting these reactions. Cloned cDNA complementary to serum albumin (SA) mRNA hybridized in accordance with single component kinetics, whereas cloned SA genomic DN...read more

Mentioned in this Paper

Nucleic Acid Hybridization Procedure
Nucleic Acids
RNA
Polyribosomes
Genomic Hybridization
Serum Albumin Measurement
Brain
Plasma Albumin
Cistron
cDNA Probes
Paper Details
References
  • References35
  • Citations22
123

Physical parameters affecting the rate and completion of RNA driven hybridization of DNA: new measurements relevant to quantitation based on kinetics

Nucleic Acids Research

Dec 20, 1982

Jeffrey Van Ness, W E Hahn

PMID: 6186985

DOI:

Abstract

Differences in the RNA-driven hybridization kinetics of genomic DNA and cDNA probes led us to examine physical parameters affecting these reactions. Cloned cDNA complementary to serum albumin (SA) mRNA hybridized in accordance with single component kinetics, whereas cloned SA genomic DN...read more

Mentioned in this Paper

Nucleic Acid Hybridization Procedure
Nucleic Acids
RNA
Polyribosomes
Genomic Hybridization

Related Papers

Paper Details
References
  • References35
  • Citations22
123

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