Physical Profile and Impact of a Calcium-Incorporated Implant Surface on Preosteoblastic Cell Morphologic and Differentiation Parameters: A Comparative Analysis

The International Journal of Oral & Maxillofacial Implants
Marco LollobrigidaAlberto De Biase

Abstract

To assess and compare topographic features and preosteoblastic cell responses of a new hydrothermally treated, calcium-incorporated surface against other commercially available implant surfaces. Four different surfaces were the subject of comparison in this study: machined (MC), resorbable blast media (RBM), sandblasted/large-grit/acid-etched (SLA), and calcium-incorporated SLA (Ca-SLA). Surface morphology and roughness were first characterized by scanning electron microscope (SEM) and white light interferometer, respectively. Preosteoblastic MC3T3-E1 cells were then cultured on the titanium surfaces. Cell morphology was observed at 24 hours, 48 hours, 7 days, and 15 days by SEM; differentiation was assessed at 7, 11, and 15 days by assaying alkaline phosphatase (ALP) activity and osteocalcin (OCN) levels. Surface characterization revealed nanotopographic features on Ca-SLA. At topographic analysis, SLA and Ca-SLA showed similar roughness values. Significant differences in cell differentiation parameters were found only at 15 days between the SLA surfaces (both Ca-incorporated and nonincorporated) and MC. Collectively, this study demonstrated that hydrothermal treatment determines the formation of nanotopography without alterin...Continue Reading

Citations

Apr 16, 2020·International Journal of Environmental Research and Public Health·Marco LollobrigidaAlberto De Biase
Aug 3, 2017·Molecular Medicine Reports·Feng ZhaiHaijun Wang
Feb 5, 2021·International Journal of Molecular Sciences·Antonio ScaranoFelice Lorusso

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