PMID: 84Dec 15, 1975

Physical properties and subunits of Haemopis grandis erythrocruorin

Biochimica Et Biophysica Acta
M R AndonianS N Vinogradov

Abstract

The erythrocruorin of the leech Haemopis grandis possessed an S20,w of 57 S at neutral pH, its isoelectric point at pH 6.0 and exhibited a slightly sigmoid oxygenation curve with n approximately 2.1 and P50 = 11.2 mm at pH 7.4. A minimum molecular weight of 24000 +/- 1500 per heme group was determined from the iron and heme contents, 0.22 +/- 0.01 and 2.73 +/- 0.14 weight %, respectively. The subunit composition of the erythrocruorin was investigated using gel filtration in sodium dodecyl sulfate and polyacrylamide gel electrophoresis in sodium dodecyl sulfate at neutral pH. Haemopis erythrocruorin dissociated in the presence of sodium dodecyl sulfate into four subunits (1 through 4) possessing molecular weights of about 27000, 23000, 21000 and 13500, respectively. When the erythrocruorin was reduced with mercaptoethanol prior to sodium dodecyl sulfate electrophoresis, three subunits were observed, possessing molecular weights of about 13000 (I), 16500 (II) and 28000 (III). Sodium dodecyl sulfate electrophoresis of the isolated subunits 1 through 4 showed that subunit I was provided by subunits 1 and 4, subunit II was provided by subunit 1 and subunit III was provided by both subunit 2 and subunit 3. Haemopis erythrocruorin thu...Continue Reading

References

Aug 15, 1975·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J M ShlomS N Vinogradov
May 15, 1973·FEBS Letters·B SalvatoF Ghiretti
Nov 28, 1966·Journal of Molecular Biology·M YamagishiK Kaziro

Citations

Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S N Vinogradov
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J LightbodyT Gotoh
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S N VinogradovD A Walz
May 5, 1990·Journal of Molecular Biology·O H KappS N Vinogradov
Jan 1, 1979·Progress in Biophysics and Molecular Biology·M C Chung, H D Ellerton

Related Concepts

Heme
Hemeproteins
Hydrogen-Ion Concentration
Iron
Leeches
2-Mercaptoethanol
Dioxygen
Protein Conformation
Irium
Macromolecular Compounds

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