PMID: 591176Oct 1, 1977

Physical properties of Tamm-Horsfall glycoprotein and its glycopolypeptide

International Journal of Peptide and Protein Research
L M Hamlin, W W Fish

Abstract

The molecular weight of the constituent glycopolypeptide chain of T-H glycoprotein was determined by sedimentation equilibrium under two entirely different sets of denaturing conditions. For both sets of denaturing conditions, the average molecular weight estimated for T-H glycopolypeptide was 74,000. The gel chromatographic behavior in 6M guanidium chloride of T-H glycopolypeptide with disulfide bonds intact as compared with its gel chromatographic behavior with disulfide bonds broken indicated that the glycopolypeptide is highly constrained by intrachain disulfide bonds.

References

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Related Concepts

Molecular Sieve Chromatography
Circular Dichroism, Vibrational
Electrophoresis
Glycopeptides
Neoglycoproteins
Spectrophotometry, Ultraviolet
Ultracentrifugation

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