PMID: 8601706Mar 28, 1996Paper

Physical state of the neuroantigen in adjuvant emulsions determines encephalitogenic status in the BALB/c mouse

Journal of Immunological Methods
J A MäättäA E Hinkkanen

Abstract

A novel form of adjuvant-neuroantigen formulation was established which was highly encephalitogenic in previously resistant BALB/c mice. The antigen formulation contained mouse whole spinal cord homogenate (MSCH), mycobacteria, and mineral oil, identically to the conventional preparation, but emulsification was completed by sonication instead of extrusion. Sonication of MSCH alone did not render a conventionally prepared emulsion encephalitogenic. The novel adjuvant formulation showed reduced water-oil droplet size, and the neuroantigen was located on the surface of the droplets as well as in the intermicellar space, while in the extruded formulation the material was buried in the droplet interior. Mice inoculated with the sonicated emulsion showed strong brain and spinal cord infiltration of lymphoid cells. The sonicated emulsion was highly encephalitogenic in all six BALB/c substrains tested. The results suggest that availability of the neuroantigen is of critical importance for the development of clinical EAE in the BALB/c mouse.

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E MerrillF M Hofman
May 1, 1988·Journal of Neuroimmunology·L X WuA Salmi
May 1, 1993·Trends in Pharmacological Sciences·F M Audibert, L D Lise

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Citations

Feb 6, 2002·Springer Seminars in Immunopathology·A Iglesias
May 12, 2007·Brain Pathology·Ikuo TsunodaRobert S Fujinami
Jan 10, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Mei DongGuojun Tan

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