PMID: 1203257Dec 19, 1975Paper

Physical studies on the binding of cis-dichlorodiamine platinum (II) to DNA and homopolynucleotides

Biochimica Et Biophysica Acta
L L Munchausen, R O Rahn

Abstract

The amount of cis-dichlorodiamine platinum (II) bound to DNAs of varying (dA + dT) content was assayed by both ultraviolet absorbance spectrophotometry and the use of the radioisotope 1 9 5 Pt. Radioisotope labeling indicates twice as much bound platinum as do optical measurements. The molar ratio of bound platinum r at saturation is approximately half the sum of the nearest-neighbor frequencies of all base-pairs that do not contain thymine. We therefore conclude that platinum does not bind to thymine in DNA. Chromatographic studies with (14C) purine-labeled DNA indicate preferential binding of platinum to guanine, followed by binding to adenine. The luminescence properties of DNA and of homopolynucleotides are strongly affected by bound platinum as a result of a heavy-atom effect. A plot of the fluorescence-to-phosphorescence ratio as a function of r gives a saturation binding curve similar to that obtained using 1 9 5 Pt. Ultraviolet irradiation of DNA treated with the platinum compound results in a 30% increase in the rate of formation of thymine homocyclobutadipyrimidine. When acetophenone sensitization is employed, platinum binding enhances cytosine homocyclobutadipyrimidine formation 10-fold presumably because the triplet...Continue Reading

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Citations

Jul 31, 2012·Bioinorganic Chemistry and Applications·Santiago Gómez-RuizGoran N Kaluđerović
Jan 15, 1980·Biochemical and Biophysical Research Communications·J P MacquetJ L Butour
Sep 14, 1978·Biochemical and Biophysical Research Communications·T J KistenmacherL G Marzilli
Sep 15, 2012·Chemistry & Biodiversity·Yuichi Shimazaki, Osamu Yamauchi
Jan 1, 1979·CRC Critical Reviews in Biochemistry·V Swaminathan, M Sundaralingam
Jan 1, 1991·Biopolymers·X Jia, L G Marzilli
Sep 1, 1977·European Journal of Biochemistry·J L Butour, J P Macquet
Nov 1, 1979·European Journal of Biochemistry·P K Ganguli, T Theophanides
Dec 16, 2011·Critical Reviews in Biotechnology·Parmjit S Panesar, John F Kennedy
May 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenjuan ZengFuyi Wang
May 29, 1981·Biochimica Et Biophysica Acta·J L Butour, J P Macquet
Oct 25, 1979·Biochimica Et Biophysica Acta·D HermannW Guschlbauer

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