Physico-chemical characterization of hamster interferon

Preparative Biochemistry
E Bollin, E Sulkowski

Abstract

The glycoprotein nature of Syrian hamster interferon was tested on several immobilized lectins. The specific retention of a small portion (20%) of interferon activity was observed only on concanavalin A-agarose; Component I of the interferon (not retained) has an apparent molecular weight of 23,500 whereas Component II (retained) is larger, 31,500. The apparent hydrophobicity of Syrian hamster interferon was probed by its chromatography on: (a) straight chain hydrocarbons of varied length; (b) aromatic ligands (aminobenzene, benzylamine, beta-phenylethylamine, gamma-phenyl-propylamine); ligands listed in (a) and (b) were immobilized to cyanogen bromide-activated agarose (isourea linkage); and (c) phenyl-agarose (Phenyl-Sepharose CL-4B), an aromatic ligand immobilized via a 2-hydroxypropyl arm to the agarose (ether linkage).

Citations

Feb 1, 1976·Journal of Virology·R TaberW Whitford
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R G Martin, S Stein
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W E StewartK Cantell
May 1, 1975·CRC Critical Reviews in Biochemistry·C N Pace
Nov 1, 1976·The Journal of General Virology·M J Morgan
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·F DornerR Weil
Jan 1, 1971·Annual Review of Biochemistry·P Cuatrecasas
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C Tanford

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