Physicochemical and Biocompatibility Properties of Type I Collagen from the Skin of Nile Tilapia (Oreochromis niloticus ) for Biomedical Applications

Marine Drugs
Wen-Kui SongHu Hou

Abstract

The aim of this study is to investigate the physicochemical properties, biosafety, and biocompatibility of the collagen extract from the skin of Nile tilapia, and evaluate its use as a potential material for biomedical applications. Two extraction methods were used to obtain acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from tilapia skin. Amino acid composition, FTIR, and SDS-PAGE results showed that ASC and PSC were type I collagen. The molecular form of ASC and PSC is (α₁)₂α₂. The FTIR spectra of ASC and PSC were similar, and the characteristic peaks corresponding to amide A, amide B, amide I, amide II, and amide III were 3323 cm-1, 2931 cm-1, 1677 cm-1, 1546 cm-1, and 1242 cm-1, respectively. Denaturation temperatures (Td) were 36.1 °C and 34.4 °C, respectively. SEM images showed the loose and porous structure of collagen, indicting its physical foundation for use in applications of biomedical materials. Negative results were obtained in an endotoxin test. Proliferation rates of osteoblastic (MC3T3E1) cells and fibroblast (L929) cells from mouse and human umbilical vein endothelial cells (HUVEC) were increased in the collagen-treated group compared with the controls. Furthermore, the acute systemic toxicity t...Continue Reading

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Citations

Jun 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chi-Hao WangSan-Lang Wang
Jul 22, 2020·Journal of the Science of Food and Agriculture·Tanyamon PetcharatSitthipong Nalinanon
Sep 27, 2020·Materials·Alina SionkowskaMagdalena Gadomska
Jun 3, 2020·Environmental Science and Pollution Research International·Zhenhua TianKang Zhang
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Jun 29, 2021·Frontiers in Bioengineering and Biotechnology·Tian ZhouXiaomeng Zhang
Aug 1, 2021·Acta histochemica·Maria Elisa Quezado Lima VerdeAna Paula Negreiros Nunes Alves

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Methods Mentioned

BETA
electrophoresis
differential scanning calorimetry
Infrared Spectroscopy

Software Mentioned

GraphPad Prism

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