Physicochemical and biological characteristics of BMP-2/IGF-1-loaded three-dimensional coaxial electrospun fibrous membranes for bone defect repair

Journal of Materials Science. Materials in Medicine
Lihua YinZhanhai Yu

Abstract

Coaxial electrospun fibrous membranes show favorable mechanical properties for use in guided bone regeneration (GBR). We used coaxial electrospinning technology to fabricate three-dimensional nanofiber membranes loaded with BMP-2 and IGF-1, and assessed the physicochemical and biological properties of these novel membranes in vitro. We fabricated four experimental groups of BMP-2/IGF-1/BSA-loaded membranes with different flow ratios (shell/core). Membrane characteristics were assessed by scanning and transmission electron microscopy, and laser confocal microscopy. Physicochemical and drug release properties were evaluated based on contact angle, mechanical property testing, X-ray diffraction analysis, and ELISA. The membranes were seeded with bone marrow-derived mesenchymal stem cells (BMMSCs) to estimate their biological properties based on cell viability and alkaline phosphatase (ALP) activity. The four membrane groups presented uniform diameters and core-shell structures. Acceleration of the shell solution flow rate increased the contact angle and mechanical properties of the fibrous membrane, while dual-factor addition did not impact fiber structure. Each drug-loaded membrane showed a gradually increasing release curve, wit...Continue Reading

References

Mar 11, 2000·Biomaterials·K Anselme
Apr 9, 2002·Clinical Orthopaedics and Related Research·Alexandre Valentin-OpranGerard E Riedel
Apr 22, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Gerhard SchmidmaierMichael Raschke
Nov 5, 2005·Journal of Biomedical Materials Research. Part a·Shyh Ming KuoTang Ching Kuan
Mar 20, 2008·The Journal of Bone and Joint Surgery. American Volume·Jeffrey O HollingerGary E Friedlaender
Apr 26, 2008·Endocrine Reviews·Andrea GiustinaErnesto Canalis
Jul 30, 2010·Clinical Oral Implants Research·Maria Retzepi, N Donos
Jul 12, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Wen Biao ZhangLim Kwong Cheung
Nov 22, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tae-Sik JangYoung-Hag Koh
Aug 17, 2012·Journal of the Royal Society, Interface·Reetta SartonevaSuvi Haimi
Dec 15, 2012·Journal of Periodontology·Momen A AtiehWarwick J Duncan
Feb 13, 2013·Injury·Taco J BlokhuisGerhard Schmidmaier
Jun 25, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Guorui JinSeeram Ramakrishna
Aug 22, 2013·Journal of Biomedical Materials Research. Part a·Zhang KuihuaMo Xiumei
Sep 27, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Janet L Crane, Xu Cao
Jan 11, 2014·International Journal of Molecular Sciences·Wei QianLu Wang
Mar 22, 2014·Colloids and Surfaces. B, Biointerfaces·Malathi SampathBalasubramanian Sengottuvelan
Apr 8, 2014·Drug Discovery Today·Daikelly I BraghirolliPatricia Pranke
May 27, 2014·Molecular Medicine Reports·Georgios D AgrogiannisAnastasia E Konstantinidou
Jun 1, 2013·Materials Today·Xianfeng WangBingyun Li
Aug 26, 2014·International Journal of Endocrinology·Vittorio Locatelli, Vittorio E Bianchi
Sep 30, 2014·Clinical Oral Implants Research·Seref EzirganliMehmet S Aydin
Jan 1, 2015·The Journal of Advanced Prosthodontics·Jwa-Young KimHye-Won Shim
Sep 1, 2015·The Journal of Clinical Endocrinology and Metabolism·N C van VarsseveldP Lips
Sep 13, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shih-Feng ChouKim A Woodrow
Jan 5, 2016·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·K DhaliwalR Farhadieh
Jan 23, 2016·Journal of Biomaterials Science. Polymer Edition·Christopher MahoneyNarayan Bhattarai
Apr 27, 2016·International Journal of Nanomedicine·Zi WangXianqun Fan

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Citations

Oct 1, 2019·Journal of Biomedical Materials Research. Part a·Lihua YinHong-Chang Lai
Nov 29, 2020·Journal of Materials Science. Materials in Medicine·Wu-Ya ChenDahong Huang
Nov 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xingzi LanYadong Tang

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