PMID: 4887194Jan 1, 1969Paper

Physicochemical characteristics of insulin secretion granules

The Biochemical Journal
H G CooreI B Täljedal

Abstract

beta-Granules were prepared from micro-dissected pancreatic islets of obese-hyperglycaemic mice. This fraction contained 60% of the insulin, 30% of the cytochrome oxidase, 16% of the acid phosphatase activity and 20% of the protein present in whole islets. The isolated granules retained a heavy metal during fractionation. Optimum conditions for granule stability were low ionic strength and pH6, the granules being unexpectedly fragile at pH7.4. The stability of the granules was unaffected by sucrose in the concentration range 50-320mm, but 1% (w/v) sodium deoxycholate released all insulin. A solubilizing effect was also noted with ATP and citrate. Spinning through 1.6m-sucrose yielded a further purification in relation to mitochondria and acid-phosphatase-carrying particles but virtually no purification in relation to protein. Electron microscopy revealed that the major contaminants were rough-surfaced vesicles and membranes. A separation of granules from acid phosphatase was achieved by phase distribution in polyethylene glycol and dextran. The location of the enzyme to the interphase was so pronounced in systems buffered with lithium phosphate that the technique may be used for future purification of acid-phosphatase-carrying ...Continue Reading

Citations

Dec 1, 1976·General and Comparative Endocrinology·W B Rhoten
Feb 1, 1982·Acta Physiologica Scandinavica·T AnderssonB Hellman
May 26, 2005·Journal of Dental Research·S-U GorrD S Darling
Oct 15, 1984·Experientia·J C Hutton
Feb 12, 1998·The Journal of Biological Chemistry·C A AspinwallJ R Lakey
Feb 24, 2007·Molecular & Cellular Proteomics : MCP·Yannick BrunnerJean-Charles Sanchez
May 1, 1989·Diabetologia·J C Hutton
Jul 1, 1971·Acta diabetologica latina·A Lernmark
May 1, 1980·Acta Physiologica Scandinavica·H Abrahamsson, E Gylfe
Apr 1, 1972·Acta Radiologica: Therapy, Physics, Biology·E I Larsson
Jul 1, 1981·Diabetologia·I B Täljedal
Jul 1, 1980·Acta Pharmacologica Et Toxicologica·H TjälveA Andersson
Jul 1, 1980·Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology·L Boquist
Mar 1, 1972·Biochemical Pharmacology·B HellmanI B Täljedal

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