PMID: 7026555Sep 25, 1981Paper

Physicochemical characterization of ribosomal particles from the eukaryote Artemia.

The Journal of Biological Chemistry
P Nieuwenhuysen, J Clauwaert

Abstract

Cytoplasmic ribosomes and their large and small subunits have been isolated from the brine shrimp Artemia, and their basic physicochemical properties have been determined. From measurements of their ratio of absorbance at 260 and 280 nm, phosphorus and protein content, buoyant density in a CsCl gradient, and density increment, values have been derived for their RNA content (49, 52, and 43 +/- 2%, respectively), absorbance coefficient at 260 nm (12.1, 2.8, and 10.5, +/- 0.4 ml/mg, respectively), and partial specific volume (0.63, 0.62, and 0.65 +/- 0.002 ml/g, respectively). Analytical boundary sedimentation has yielded their standard sedimentation coefficient (81, 59, and 38, +/- 1 S, respectively). Photon correlation spectroscopy of laser light scattered from centrifuged solutions has yielded their translational diffusion coefficients (1.41 +/- 0.02, 1.61 +/- 0.03, and 1.80 +/- 0.04 X 10(-7) cm2/s, respectively). Combinations of these data have yielded values for their molecular weight (3.85 +/- 0.2, 2.45 +/- 0.2, and 1.45 +/- 0.1 X 10(6), respectively), for the total molecular weight of the free acid form of their RNA (1.9 +/- 0.1, 1.27 +/- 0.08, and 0.62 +/- 0.04 X 10(6), respectively) and of their proteins (2.0 +/- 0.1, 1.2...Continue Reading

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