Physicochemical evolution and molecular adaptation of the cetacean osmoregulation-related gene UT-A2 and implications for functional studies

Scientific Reports
Jingzhen WangDing Wang

Abstract

Cetaceans have an enigmatic evolutionary history of re-invading aquatic habitats. One of their essential adaptabilities that has enabled this process is their homeostatic strategy adjustment. Here, we investigated the physicochemical evolution and molecular adaptation of the cetacean urea transporter UT-A2, which plays an important role in urine concentration and water homeostasis. First, we cloned UT-A2 from the freshwater Yangtze finless porpoise, after which bioinformatics analyses were conducted based on available datasets (including freshwater baiji and marine toothed and baleen whales) using MEGA, PAML, DataMonkey, TreeSAAP and Consurf. Our findings suggest that the UT-A2 protein shows folding similar to that of dvUT and UT-B, whereas some variations occurred in the functional So and Si regions of the selectivity filter. Additionally, several regions of the cetacean UT-A2 protein have experienced molecular adaptations. We suggest that positive-destabilizing selection could contribute to adaptations by influencing its biochemical and conformational character. The conservation of amino acid residues within the selectivity filter of the urea conduction pore is likely to be necessary for urea conduction, whereas the non-conse...Continue Reading

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Citations

Aug 18, 2016·Molecular Phylogenetics and Evolution·Megan L PorterJulian C Partridge
Jul 24, 2018·Molecular Ecology Resources·Marijke AutenriethRalph Tiedemann
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Vivien J ChebiiJean-Baka Domelevo Entfellner

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Datasets Mentioned

BETA
AB266067
AB266069
AB266070
AF367359

Methods Mentioned

BETA
X-ray
PCR

Software Mentioned

MEGA4
PAML
MrBayes
BioEdit
DNA Star Seq Man
ConSurf
dvUT
DataMonkey
codeml ( PAML )
CLUSTAL X

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