Physicochemical factors influencing bacterial transfer from contact lenses to surfaces with different roughness and wettability

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Pit B J VermeltfoortG M Bruinsma

Abstract

The aim of this study was to determine the transfer of Pseudomonas aeruginosa No. 3 and Staphylococcus aureus 835 from contact lenses to surfaces with different hydrophobicity and roughness. Bacteria were allowed to adhere to contact lenses (Surevue, PureVision, or Focus Night & Day) by incubating the lenses in a bacterial suspension for 30 min. The contaminated lenses were put on a glass, poly(methylmethacrylate), or silicone rubber substratum, shaped to mimic the eye. After 2 and 16 h, lenses were separated from the substrata and bacteria were swabbed off from the respective surfaces and resuspended in saline. Appropriate serial dilutions of these suspensions were made, from which aliquots were plated on agar for enumeration. Bacterial transfer varied between 4 and 60%, depending on the combination of strain, contact time, contact lens, and substratum surface. For P. aeruginosa No. 3, transfer was significantly higher after 16 h than after 2 h, whereas less increase with time was seen for S. aureus 835. Bacterial transfer from all tested contact lenses was least to silicone rubber, the most hydrophobic and roughest substratum surface included.

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Citations

May 7, 2010·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Maria J GiraldezEva Yebra-Pimentel
Jan 21, 2014·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Ka Yin ChanMaureen Boost
Sep 22, 2015·Microscopy Research and Technique·Ştefan ŢăluMustafa Oguzhan Caglayan
Jul 12, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·J M González-MéijomeM A Parafita
Nov 14, 2018·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Shamaila TahirKaren Vickery
Oct 24, 2006·Journal of Biomedical Materials Research. Part a·Bas A S KnobbenHenk J Busscher
Oct 25, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M LiraM Elisabete C D Real Oliveira
Apr 9, 2010·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Kara L Menzies, Lyndon Jones
Apr 9, 2010·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Maria J GiraldezEva Yebra-Pimentel
May 18, 2010·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Maria J GiraldezEva Yebra-Pimentel
May 5, 2020·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Fiona Stapleton
Apr 24, 2014·Eye & Contact Lens·Madalena LiraManuel F M Costa

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