Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection

Biopolymers
Ali NamvarFatemeh Motevalli

Abstract

Delivery of the macromolecules including DNA, miRNA, and antisense oligonucleotides is typically mediated by carriers due to the large size and negative charge. Different physical (e.g., gene gun or electroporation), and chemical (e.g., cationic polymer or lipid) vectors have been already used to improve the efficiency of gene transfer. Polymer-based DNA delivery systems have attracted special interest, in particular via intravenous injection with many intra- and extracellular barriers. The recent progress has shown that stimuli-responsive polymers entitled as multifunctional nucleic acid vehicles can act to target specific cells. These nonviral carriers are classified by the type of stimulus including reduction potential, pH, and temperature. Generally, the physicochemical characterization of DNA-polymer complexes is critical to enhance the transfection potency via protection of DNA from nuclease digestion, endosomal escape, and nuclear localization. The successful clinical applications will depend on an exact insight of barriers in gene delivery and development of carriers overcoming these barriers. Consequently, improvement of novel cationic polymers with low toxicity and effective for biomedical use has attracted a great at...Continue Reading

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Citations

Feb 2, 2017·Drug Development and Industrial Pharmacy·Oscar Escalona-RayoDavid Quintanar-Guerrero
Jan 13, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mian WangHaijie Yang
Feb 18, 2021·Advanced Materials·Dorsa Morshedi RadMajid Ebrahimi Warkiani
Mar 26, 2021·Malaria Journal·António B MapossaTaneshka Kruger
May 8, 2021·Computational Biology and Chemistry·Anjan Kumar PayraAnupam Ghosh

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