Physicochemical properties of the Ljungan virus prototype virion in different environments: inactivated by heat but resistant to acidic pH, detergents and non-physiological environments such as Virkon-containing solutions

Microbiology and Immunology
Jens-Ola EkströmA Michael Lindberg

Abstract

It is of great importance to know how a virus particle is affected by environmental conditions. Physicochemical properties of the virion will affect the virus viability in different environments, viral transmission between hosts, and will also be important for safe handling of the virus. The physicochemical properties of the Ljungan virus (LV) prototype, 87-012, adapted to grow in cell culture were evaluated using both LV in crude cell extracts and purified virions. Replication of LV was completely inhibited by heat. Titers of LV were unaffected by acidic pH, reduced but not completely abolished by alkaline pH, and unaffected by exposure to the detergents Triton X-100 and SDS. Surprisingly, viable LV was still detected after incubation in the acidic, oxidising and detergent-containing environment produced by the commonly used disinfectant Virkon. In conclusion, LV is resilient to extreme pH, detergents and also to oxidising environments, but is sensitive to heat treatment.

References

Apr 1, 1976·Journal of Wildlife Diseases·A J MainR C Wallis
Feb 1, 1975·Experimental and Molecular Pathology·C M Trant, R M Jamison
May 1, 1990·The Journal of Hospital Infection·R TylerC Bradley
Jan 1, 1974·Archiv für die gesamte Virusforschung·R M Jamison
Nov 1, 1966·Virology·R B ArlinghausG F Vande Woude
Jan 1, 1996·Scandinavian Journal of Infectious Diseases·A Ehrnst, M Eriksson
Jan 1, 1996·Archives of Virology·D SchnurrJ Connor
Feb 20, 1999·Die Naturwissenschaften·B BeerA Bukreyev
Feb 2, 2000·Reviews in Medical Virology·G StanwayT Hyypiä
Mar 13, 2001·The Journal of Infection·K HayashiJ Takamatsu
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·N Dejucq, B Jégou
Apr 17, 2002·Virus Research·A Michael Lindberg, Susanne Johansson
Mar 26, 2003·The Journal of General Virology·E Susanne JohanssonA Michael Lindberg
May 15, 2003·International Journal of Experimental Diabesity Research·Bo NiklassonA Lernmark
Apr 1, 1962·Virology·C WALLIS, J L MENICK
Apr 20, 2004·Biochemical and Biophysical Research Communications·E Susanne JohanssonA Michael Lindberg
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Austin L Hughes
Aug 9, 2006·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Annika SamsioeBo Niklasson
Mar 6, 2007·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz

Citations

Oct 9, 2015·Nature Communications·Ling ZhuDavid I Stuart
Jun 4, 2020·Vector Borne and Zoonotic Diseases·Cristina FevolaHeidi C Hauffe

Related Concepts

Monoperoxysulfate, disodium salt
Metazoa
Synthetic Detergent
Biocides
Hot Temperature
Hydrogen-Ion Concentration
Oxidation-Reduction
Peroxides
Irium
Sulfuric Acids

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Birth Defects Research. Part B, Developmental and Reproductive Toxicology
Annika SamsioeWilliam Klitz
Birth Defects Research. Part A, Clinical and Molecular Teratology
Bo NiklassonWilliam Klitz
Birth Defects Research. Part B, Developmental and Reproductive Toxicology
Annika SamsioeBo Niklasson
Forensic Science, Medicine, and Pathology
Bo NiklassonWilliam Klitz
© 2021 Meta ULC. All rights reserved