PMID: 6983365Oct 26, 1982Paper

Physicochemical studies of pregnancy-specific beta 1-glycoprotein: unusual ultracentrifugal and circular dichroic properties

Biochemistry
J C OsborneH Bohn

Abstract

The molecular properties of native and modified pregnancy-specific beta 1-glycoprotein (SP1) from human placenta were evaluated by sedimentation equilibrium, gel electrophoresis, and circular dichroic measurements. Native SP1 contained 6.2% N-acetylneuraminic acid (NANA), 5.8% galactose (Gal), 13% N-acetylglucosamine, 6.5% mannose, and 1.1% fucose but no detectable N-acetylgalactosamine. Treatment with mixed exoglycosidases and alpha-mannosidase removed 79% of the carbohydrate including all of the NANA and Gal. The intensity of the circular dichroic spectrum of SP1 in the far ultraviolet was quite low with a positive maximum at 235 nm and a negative maximum at 215 nm. The 235-nm band was lost upon treatment with reducing agents or with guanidinium chloride (GdmCl), but not by treatment with neuraminidase. Treatment of SP1 with neuraminidase, or with mixed exoglycosidases and alpha-mannosidase, resulted in decreases of the apparent molecular weight obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Neither exposure of SP1 to GdmCl nor its reduction and alkylation resulted in the appearance of subunits on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The partial specific volume of SP1 determined e...Continue Reading

References

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Citations

Jan 1, 1988·Molecular Biology Reports·J L BoccoL C Patrito
Apr 1, 1987·Biulleten' eksperimental'noĭ biologii i meditsiny·S K KrivonosovA D Efremov
May 30, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·S W RosenJ C Osborne
Sep 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·A PalaL Carenza
Nov 1, 1985·Journal of Reproductive Immunology·U W Mueller, W R Jones
Dec 1, 1985·Archives of Biochemistry and Biophysics·H H VarnerA T Hewitt

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