Physicochemical Tools for Visualizing and Quantifying Cell-Generated Forces.

ACS Chemical Biology
Ashley K Nguyen, Kristopher A Kilian

Abstract

To discern how mechanical forces coordinate biological outcomes, methods that map cell-generated forces in a spatiotemporal manner, and at cellular length scales, are critical. In their native environment, whether it be within compact multicellular three-dimensional structures or sparsely populated fibrillar networks of the extracellular matrix, cells are constantly exposed to a slew of physical forces acting on them from all directions. At the same time, cells exert highly localized forces of their own on their surroundings and on neighboring cells. Together, the generation and transmission of these forces can control diverse cellular activities and behavior as well as influence cell fate decisions. To thoroughly understand these processes, we must first be able to characterize and measure such forces. However, our experimental needs and technical capabilities are in discord-while it is apparent that we should study cell-generated forces within more biologically relevant 3D environments, this goal remains challenging because of caveats associated with complex "sensing-transduction-readout" modalities. In this Review, we will discuss the latest techniques for measuring cell-generated forces. We will highlight recent advances in...Continue Reading

References

Dec 1, 1994·The Journal of Cell Biology·J LeeK Jacobson
Dec 1, 1995·Journal of Biomechanics·P F DaviesK A Barbee
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·N C Seeman
Mar 30, 1999·Biophysical Journal·M Dembo, Y L Wang
Nov 27, 2001·Science·E CukiermanK M Yamada
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John L TanChristopher S Chen
Jan 8, 2004·Annals of Medicine·Donald E Ingber
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olivia du RoureBenoit Ladoux
Oct 21, 2005·Bio Systems·Ulrich S SchwarzIlka B Bischofs
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nathan J SniadeckiChristopher S Chen
Sep 11, 2007·Biophysical Journal·Benedikt SabassUlrich S Schwarz
Jun 28, 2008·Current Opinion in Cell Biology·Martin A Schwartz, Douglas W DeSimone
Sep 9, 2008·Biophysical Journal·Cynthia A Reinhart-KingDaniel A Hammer
Jan 27, 2009·Biophysical Journal·Christopher A LemmonLewis H Romer
Jun 3, 2010·The European Physical Journal. E, Soft Matter·N B BeckerR Everaers
Nov 16, 2010·Nature Methods·Wesley R LegantChristopher S Chen
Jan 26, 2011·Optics Express·Zhuo WangGabriel Popescu
Aug 19, 2011·Progress in Biophysics and Molecular Biology·D D ChanC P Neu
Nov 1, 2011·Nature Methods·Daniel R StableyKhalid S Salaita
Nov 29, 2011·Cell·Peter Friedl, Stephanie Alexander
Nov 30, 2011·The Journal of Cell Biology·Kimihide HayakawaMasahiro Sokabe
Mar 6, 2012·Journal of Cell Science·Fanjie Meng, Frederick Sachs
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Saba GhassemiJames Hone
Apr 6, 2012·PloS One·Thorsten M KochBen Fabry
May 2, 2012·The Journal of Cell Biology·Deok-Ho KimAndre Levchenko
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nicolas BorghiAlexander R Dunn
Sep 1, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Raymond H W LamJianping Fu
Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wesley R LegantChristopher S Chen
Mar 19, 2013·Journal of the American Chemical Society·Yang LiuKhalid Salaita
Oct 9, 2013·Lab on a Chip·Phrabha S RamanKonstantinos Konstantopoulos
Dec 10, 2013·Nature Methods·Otger CampàsDonald E Ingber
Apr 30, 2014·The Journal of Cell Biology·Ayelet LesmanGuruswami Ravichandran

❮ Previous
Next ❯

Citations

May 1, 2021·Cells·Veronika PfannenstillMarco Fritzsche

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.