Physiochemical characterization of lymphocyte inhibitory factor (LyIF) isolated from avian B and T cells

Cellular Immunology
D E Martin, B Glick


Thymic (T) or bursal (B) lymphocytes from chicks sensitized to Mycobacterium tuberculosis produce an avian lymphocyte inhibitory factor (LyIF). The physiochemical properties of both T and B LyIF were established by ultrafiltration which yielded four fractions with molecular weight ranges of greater than 100,000; 50,000-100,000; 10,000-50,000; and less than 10,000; enzymatic treatment with chymotrypsin and neuraminidase; varying pH; and heat exposure. These studies demonstrated that the maximum activity for both T and B LyIF was within a molecular weight range of 10,000-50,000. Both were sensitive to chymotrypsin and neuraminidase treatment. Both were stable at 56 degrees C for 30 min and resistant to changes in pH from 5 to 9. T-Cell migration was inhibited equally by B or T LyIF, while B-cell migration was inhibited to a lesser extent by T LyIF and B LyIF. Further experiments should establish the reasons for these observed differences in cross-reactivity.


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Oct 1, 1973·The Journal of Experimental Medicine·T YoshidaS Cohen
Jun 1, 1980·General and Comparative Endocrinology·M D Livezey, R S Sundick


Jan 1, 1990·Developmental and Comparative Immunology·P Joshi, B Glick
Mar 1, 1987·Veterinary Immunology and Immunopathology·H Weiler, V Von Bülow
Feb 26, 2000·Developmental and Comparative Immunology·C Siatskas, R L Boyd
Dec 1, 1984·The Journal of Experimental Zoology·B Glick

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