Physiochemical characterization of the nisin-membrane interaction with liposomes derived from Listeria monocytogenes.

Applied and Environmental Microbiology
K WinkowskiThomas J Montville

Abstract

Mechanistic information about the bacteriocin nisin was obtained by examining the efflux of 5(6)-carboxy-fluorescein from Listeria monocytogenes-derived liposomes. The initial leakage rate (percentage of efflux per minute) of the entrapped dye was dependent on both nisin and lipid concentrations. At all nisin concentrations tested, 5(6)-carboxyfluorescein efflux plateaued before all of the 5(6)-carboxyfluorescein was released (suggesting that pore formation was transient), but efflux resumed when more nisin was added. Isotherms for the binding of nisin to liposomes constructed on the basis of the Langmuir isotherm gave an apparent binding constant of 6.2 x 10(5)M(-1) at pH 6.0. The critical number of nisin molecules required to induce efflux from liposomes at pH 6.0 was approximately 7,000 molecules per liposome. The pH affected the 5(6)-carboxyfluorescein leakage rates, with higher pH values resulting in higher leakage rates. The increased leakage rate observed at higher pH values was not due to an increase in the binding affinity of the nisin molecules towards the liposomal membrane. Rather, the critical number of nisin molecules required to induce activity was decreased (approximately 1,000 nisin molecules per liposome at pH...Continue Reading

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Citations

Apr 3, 2001·Applied and Environmental Microbiology·C HerranzM L Chikindas
Feb 2, 2000·Applied and Environmental Microbiology·L NilssonT J Montville
Sep 8, 2006·Applied and Environmental Microbiology·Sikder M AsaduzzamanKenji Sonomoto
Aug 21, 2007·Applied and Environmental Microbiology·Mohamed Badaoui NajjarThomas J Montville
Dec 2, 2009·Applied and Environmental Microbiology·Mariano Prado-AcostaCarmen Sanchez Rivas
Apr 10, 1999·Letters in Applied Microbiology·T J MontvilleY Chen
Jun 22, 2006·Journal of Food Protection·M Al-HolyB Rasco
Mar 4, 1999·Journal of Applied Microbiology·M MansourJ B Milliere
Aug 22, 2012·Journal of Applied Microbiology·A A HugoP F Pérez
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Feb 11, 2005·Chemical Reviews·Champak ChatterjeeWilfred A van der Donk
Feb 13, 2008·Biochemistry·Leif SmithRavi S Orugunty
Jul 2, 1998·Biochemical and Biophysical Research Communications·G A DykesJ W Hastings
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Oct 19, 2006·Journal of Bioscience and Bioengineering·Jun-ichi NagaoKenji Sonomoto

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