PMID: 321337Jan 1, 1977Paper

Physiochemical consequences of opsonization of Salmonella typhimurium with hyperimmune IgG and complement

Immunology
O StendahlL Edebo

Abstract

Partition in an aqueous, two-polymer phase system containing dextran and polyethylene glycol was employed to investigate the physico-chemical changes inflicted upon the cell surface of a smooth strain of Salmonella typhimurium by the binding of antibody IgG and complement. The minimum antibody concentration for increased phagocytosis in vitro was approximately the same as that for a significant change in two-phase partition, ca 8000 mol/bacterium, whereas a lower concentration, less than 4000 mol/bacterium, was sufficient to increase clearance in vivo. After pepsin digestion of IgG, larger quantities, ca 35,000 mol/bacterium, was required for opsonization and to influence two-phase partition. Addition of normal rabbit or guinea-pig serum to bacteria sensitized with a low concentration of antibody IgG conspicuously enhanced phagocytosis and affinity for the dextran-rich phase. The results show that binding of 8000 IgG antibody molecules or more to smooth S. typhimurium generates physicochemical changes of the bacterial surface which from studies on S leads to R mutations are known to correlate with hydrophobicity, negative charge and phagocytosis. Such results support the view that one important function of IgG antibody and comp...Continue Reading

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