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Physiochemical studies on interactions between DNA and RNA polymerase. Unwinding of the DNA helix by Escherichia coli RNA polymerase

Nucleic Acids Research

Jan 1, 1977

Jade C WangJenifer M Saucier

PMID: 331252

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Abstract

In a medium containing 10mM Tris, pH 8, 10 mM MG++, 50 mM K+ and 10 mM NH4, the binding of an E. coli RNA polymerase holoenzyme unwinds the DNA helix by about 240 degrees at 37 degrees C. In this medium the total unwinding of the DNA increases linearly with the molar ratio of polymerase...read more

Mentioned in this Paper

Potassium
RNA Conformation
Polynucleotide Ligases
Transcriptase
DNA, Viral
Osmolality
Coliphages
Alkalescens-Dispar Group
Paper Details
References
  • References20
  • Citations51
12
  • References20
  • Citations51

Physiochemical studies on interactions between DNA and RNA polymerase. Unwinding of the DNA helix by Escherichia coli RNA polymerase

Nucleic Acids Research

Jan 1, 1977

Jade C WangJenifer M Saucier

PMID: 331252

DOI:

Abstract

In a medium containing 10mM Tris, pH 8, 10 mM MG++, 50 mM K+ and 10 mM NH4, the binding of an E. coli RNA polymerase holoenzyme unwinds the DNA helix by about 240 degrees at 37 degrees C. In this medium the total unwinding of the DNA increases linearly with the molar ratio of polymerase...read more

Mentioned in this Paper

Potassium
RNA Conformation
Polynucleotide Ligases
Transcriptase
DNA, Viral

Related Papers

Paper Details
References
  • References20
  • Citations51
12
  • References20
  • Citations51

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