Physiologic electrical stimulation provokes intracellular calcium increase mediated by phospholipase C activation in human osteoblasts

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Layla KhatibMichael Cho

Abstract

Strong exogenous electrical stimulation (ES) can induce changes in intracellular calcium ion concentration ([Ca2+]i). It remains to be elucidated, however, whether physiologically relevant ES (e.g., 1-2 V/cm) could alter [Ca2+]i. We have used fluorescence microscopy to quantify [Ca2+]i changes in response to direct current (dc) ES in human fetal osteoblasts. Increases in [Ca2+]i in response to 2 V/cm ES show a noticeable (20-min) time delay, followed by a 45-fold rise from the baseline of 40 nM to 1.8 microM. Treatment of cells with verapamil does not affect ES-induced [Ca2+]i increases, but inhibition of phospholipase C (PLC) does prevent such increases, which suggests that receptor-regulated release of Ca2+ from intracellular stores is likely to be involved. Treatment of cells with the stretch-activated cation channel (SACC) blocker Gd3+ partially inhibits ES-induced [Ca2+]i increases, as does chelation of intracellular Ca2+. These results are consistent with a model in which physiologically relevant ES does not activate voltage-gated Ca2+ channels (VGCCs) directly, but rather stimulates PLC-coupled cell surface receptors that induce [Ca2+]i increases by activating IP3-dependent intracellular processes. The Ca2+ influx that f...Continue Reading

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Citations

Oct 27, 2006·Annals of Biomedical Engineering·Ayman HamedMichael Cho
Aug 1, 2007·Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)·F Bistolfi
Apr 10, 2010·Journal of Biomedicine & Biotechnology·Igor TitushkinMichael Cho
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Feb 21, 2019·Journal of Biomedical Materials Research. Part a·Wei JingWeihong Zhong
Feb 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shan SunMichael Cho
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Sep 22, 2007·Electromagnetic Biology and Medicine·Xiaojun ZhangJun Wen
Nov 9, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shaobo CaiElizabeth G Loboa
Apr 25, 2017·Advanced Materials·Gonzalo MurilloJaume Esteve
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Apr 24, 2020·Annals of Biomedical Engineering·Caleb LiebmanMichael Cho
Sep 14, 2021·Frontiers of Medicine·Sijin LiYao Sun

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