Physiologic Mechanisms Involved in Accumulation of 3-Hydroxypropionaldehyde during Fermentation of Glycerol by Enterobacter agglomerans.

Applied and Environmental Microbiology
F BarbiratoA Bories

Abstract

When grown in 700 mM glycerol within the pH range 6.0 to 7.5, anaerobic pH-regulated cultures of Enterobacter agglomerans exhibited an extracellular accumulation of 3-hydroxypropionaldehyde (3-HPA). This phenomenon, which causes fermentation cessation, occurred earlier when pH was low. In contrast, substrate consumption was complete at pH 8. Levels of glycerol-catabolizing enzymes, i.e., glycerol dehydrogenase and dihydroxyacetone kinase for the oxidative route and glycerol dehydratase and 1,3-propanediol dehydrogenase for the reductive route, as well as the nucleotide pools were determined periodically in the pH 7- and pH 8-regulated cultures. A NAD/NADH ratio of 1.7 was correlated with the beginning of the production of the inhibitory metabolite. Further accumulation was dependent on the ratio of glycerol dehydratase activity to 1,3-propanediol dehydrogenase activity. For a ratio higher than 1, 3-HPA was produced until fermentation ceased, which occurred for the pH 7-regulated culture. At pH 8, a value below 1 was noticed and 3-HPA accumulation was transient, while the NAD/NADH ratio decreased. The low rate of glycerol dissimilation following the appearance of 3-HPA in the culture medium was attributed to the strong inhibitor...Continue Reading

References

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Citations

Aug 8, 2008·Journal of Industrial Microbiology & Biotechnology·Jian HaoDehua Liu
Feb 13, 2013·World Journal of Microbiology & Biotechnology·Douglas A S GrahameTakuji Tanaka
Apr 9, 2009·Applied Microbiology and Biotechnology·Kouta Hatayama, Tatsuo Yagishita
Sep 26, 2013·Biotechnology Letters·Thomas P West, Jessica L Peterson
Mar 12, 2016·Biotechnology for Biofuels·Wei JiangBaishan Fang
May 28, 2010·Biotechnology and Bioengineering·Afroditi ChatzifragkouSeraphim Papanikolaou
Mar 5, 2016·PloS One·Marinalva Martins-PinheiroCarlos F M Menck
Oct 17, 2008·Chemical Reviews·Yuguo ZhengYinchu Shen
Aug 12, 2009·Biotechnology Advances·R K SaxenaJasmine Isar

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