PMID: 2499202May 1, 1989Paper

Physiological activation of the mitochondrion and the transformation capacity of DFMO induced intermediate and short stumpy bloodstream form trypanosomes

The American Journal of Tropical Medicine and Hygiene
B F Giffin, P P McCann

Abstract

Bloodstream forms of Trypanosoma brucei brucei (EATRO 110) were cultured with 100 microM difluoromethylornithine (DFMO). After 48 hr, intracellular putrescine was depleted and cells were positive when histochemically stained for the mitochondrial marker enzyme, NAD diaphorase, and exhibited mitochondrial proliferation and cristae development when examined by electron microscopy. This suggested that the mitochondrion was undergoing the physiological transformation necessary for successful transmission of the bloodstream form to the vector, namely the initiation of development of a TCA cycle and cytochrome system. The short stumpy forms that appeared by day 4 of culture, although physiologically transformed, were not viable in so far as they were not capable of transforming to procyclic trypomastigotes when introduced into SDM-79 medium. When rats infected with T. b. brucei were given 4% (w/v) DFMO in their drinking water, they were cured within 72 hr. Trypanosomes removed from animals and stained for NAD diaphorase showed mitochondrial transformation, as well as an intermediate and short stumpy morphology, at 36 and 60 hr, respectively. Data from this study on the growth and transformation characteristics of the DFMO induced int...Continue Reading

Citations

May 1, 1993·The Journal of Eukaryotic Microbiology·A A DivoA C Sartorelli
Dec 27, 1990·European Journal of Biochemistry·H Lohrer, R L Krauth-Siegel
Sep 1, 1990·Transactions of the Royal Society of Tropical Medicine and Hygiene·A H Fairlamb
May 18, 1999·The Journal of Biological Chemistry·B M BakkerH V Westerhoff
Aug 15, 1993·European Journal of Biochemistry·E J BienenA B Clarkson
Jun 21, 2019·FEMS Microbiology Reviews·Priscilla NgothoMatthias Marti
Apr 11, 1991·Molecular and Biochemical Parasitology·E J BienenA B Clarkson
Sep 1, 1991·Molecular and Biochemical Parasitology·E J Bienen, M K Shaw
Sep 1, 1993·Acta Tropica·C J Bacchi, N Yarlett
Jun 12, 2003·Parasitology International·Yoshisada YabuNobuo Ohta

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