PMID: 11606194Oct 19, 2001Paper

Physiological and biochemical characteristics of poly gamma-glutamate synthetase complex of Bacillus subtilis

European Journal of Biochemistry
Makoto AshiuchiHaruo Misono

Abstract

An enzymatic system for poly gamma-glutamate (PGA) synthesis in Bacillus subtilis, the PgsBCA system, was investigated. The gene-disruption experiment showed that the enzymatic system was the sole machinery of PGA synthesis in B. subtilis. We succeeded in achieving the enzymatic synthesis of elongated PGAs with the cell membrane of the Escherichia coli clone producing PgsBCA in the presence of ATP and D-glutamate. The enzyme preparation solubilized from the membrane with 8 mM Chaps catalyzed ADP-forming ATP hydrolysis only in the presence of glutamate; the D-enantiomer was the best cosubstrate, followed by the L-enantiomer. Each component of the system, PgsB, PgsC, and PgsA, was translated in vitro and the glutamate-dependent ATPase reaction was kinetically analyzed. The PGA synthetase complex, PgsBCA, was suggested to be an atypical amide ligase.

References

May 1, 1992·Applied Microbiology and Biotechnology·T HaraS Ueda
Aug 1, 1985·Infection and Immunity·B D GreenB E Ivins
Nov 15, 1997·Bioscience, Biotechnology, and Biochemistry·K AbeY Asada
Jun 5, 1999·Journal of Molecular Biology·J A BertrandO Dideberg
Jan 11, 2000·Archives of Microbiology·E AboulmagdA Steinbüchel
May 9, 2001·Applied Microbiology and Biotechnology·J Nielsen
Feb 1, 1956·Journal of General Microbiology·S GUEX-HOLZER, J TOMCSIK
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Jan 1, 1946·The Biochemical Journal·W E HANBY, H N RYDON
Jan 1, 1993·Bioscience, Biotechnology, and Biochemistry·H KubotaM Taniguchi

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Citations

Nov 10, 2005·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Moon-Hee SungMakoto Ashiuchi
Mar 13, 2013·Journal of Industrial Microbiology & Biotechnology·Iván Muñoz-Gutiérrez, Alfredo Martinez
Jun 19, 2009·The Journal of Biological Chemistry·Ruiying WuAndrzej Joachimiak
Sep 3, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Zui FujimotoKeitarou Kimura
Oct 7, 2004·Applied and Environmental Microbiology·Dongru QiuMitsuhiro Itaya
Jul 9, 2004·Applied and Environmental Microbiology·Makoto AshiuchiHaruo Misono
Apr 7, 2006·Applied and Environmental Microbiology·Masanobu Nishikawa, Ken'ichi Ogawa
Apr 11, 2007·Infection and Immunity·Jingliang SuJing-Ren Zhang
Jul 25, 2006·Bioscience, Biotechnology, and Biochemistry·Makoto AshiuchiHaruo Misono
Mar 25, 2014·Applied and Environmental Microbiology·Bai-fen SongLi-jie Tang
Aug 12, 2014·Applied Microbiology and Biotechnology·Jan SchüürmannJoachim Jose
Aug 28, 2007·Biochemical and Biophysical Research Communications·Makoto AshiuchiHaruo Misono
Nov 19, 2003·Biochemical and Biophysical Research Communications·Takahiro KawaiYoshikazu Izumi
May 13, 2006·Molecular Microbiology·Thomas Candela, Agnès Fouet
Jan 25, 2006·Molecular Microbiology·Steven S BrandaRoberto Kolter
Apr 14, 2016·BioMed Research International·Biaosheng LinMaochun Luo
Oct 8, 2014·Microbiology·Adetoro OgunleyeIza Radecka
Sep 3, 2014·Journal of Biomaterials Science. Polymer Edition·Sung-Bin ParkHiroshi Uyama
Apr 7, 2018·World Journal of Microbiology & Biotechnology·Osamu KuritaHiroyuki Iwamoto
Sep 3, 2010·Biotechnology and Bioengineering·Daisuke YamashiroMakoto Ashiuchi
Apr 29, 2006·International Journal of Cancer. Journal International Du Cancer·Haryoung PooSeung-Hoon Lee
Aug 26, 2010·FEMS Microbiology Letters·Massimiliano MarvasiLilliam Casillas Martinez
Jul 17, 2013·Microbial Biotechnology·Makoto Ashiuchi
Jul 3, 2013·Natural Product Reports·Yoshimitsu HamanoKuniki Kino

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