Physiological and molecular characterization of connexin hemichannels in zebrafish retinal horizontal cells.

Journal of Neurophysiology
Ziyi SunDouglas G McMahon

Abstract

Connexin channels mediate electrical synaptic transmission when assembled as cell-to-cell pores at gap junctions and can mediate transmembrane currents when expressed in plasma membranes as hemichannels. They are widely expressed in the vertebrate retina where in electrical synapses they are critical for transmission of visual signals. While the roles of connexins in electrical synapses are well-studied, the function and roles of connexin hemichannels in the nervous system are less well understood. Genetic deletion in zebrafish of connexin (Cx) 55.5 alters horizontal cell feedback to cones, spectral responses, and visual behavior. Here, we have characterized the properties of hemichannel currents in zebrafish retinal horizontal cells and examined the roles of two connexin isoforms, Cx55.5 and Cx52.6, that are coexpressed in these cells. We report that zebrafish horizontal cells express hemichannel currents that conduct inward current at physiological negative potentials and Ca(2+) levels. Manipulation of Cx55.5 and Cx52.6 gene expression in horizontal cells of adult zebrafish revealed that both Cx55.5 and Cx52.6 contribute to hemichannel currents; however, Cx55.5 expression is necessary for high-amplitude currents. Similarly, c...Continue Reading

References

Jan 1, 1992·The Journal of Physiology·S H DeVries, E A Schwartz
Apr 25, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·V K VerselisF F Bukauskas
Aug 24, 2000·The Journal of Comparative Neurology·M GüldenagelK Willecke
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·D Q Zhang, D G McMahon
May 12, 2001·Science·M KamermansR Weiler
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SrinivasD C Spray
Mar 28, 2002·Biophysical Journal·Derek L Beahm, James E Hall
Sep 25, 2002·Physiological Reviews·E A Schwartz
Dec 19, 2003·Cell Communication & Adhesion·Stephen C MasseyJohn O'Brien
Apr 20, 2004·Cardiovascular Research·Goran Söhl, Klaus Willecke
Oct 16, 2004·Cellular and Molecular Neurobiology·Harris RippsJane Zakevicius
Apr 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John P VesseySteven Barnes
May 13, 2006·Science·Roger J ThompsonBrian A MacVicar
Feb 15, 2007·The Journal of Comparative Neurology·Colleen R ShieldsMaarten Kamermans
Dec 29, 2007·Developmental Neurobiology·Ryan ThummelDavid R Hyde
Jul 24, 2008·Cell Communication & Adhesion·Georg ZoidlRolf Dermietzel
Jul 24, 2008·Glia·M L CotrinaM Nedergaard
May 15, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marjelle VanLeeuwenMaarten Kamermans
Jun 6, 2009·Nature Reviews. Neuroscience·Stewart A Bloomfield, Béla Völgyi
Mar 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiuxin LiuPasko Rakic

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Citations

Oct 14, 2014·Frontiers in Cellular Neuroscience·Sarah KurtenbachGeorg Zoidl
Nov 20, 2014·Frontiers in Cellular Neuroscience·Giselle CheungNathalie Rouach
May 11, 2016·The Journal of Physiology·Jennifer OltWalter Marcotti
Oct 30, 2016·Neuroscience·H GrebU Janssen-Bienhold
Jan 15, 2013·Progress in Retinal and Eye Research·Béla VölgyiRóbert Gábriel
Nov 11, 2016·Journal of Neurophysiology·Michael W Country, Michael G Jonz
Jun 12, 2018·Annual Review of Vision Science·John O'Brien, Stewart A Bloomfield
Mar 13, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Cristina M NathaGuillermo A Altenberg

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