Physiological and pharmacological modulation of the embryonic skeletal muscle calcium channel splice variant CaV1.1e

Biophysical Journal
Bruno BenedettiBernhard E Flucher

Abstract

CaV1.1e is the voltage-gated calcium channel splice variant of embryonic skeletal muscle. It differs from the adult CaV1.1a splice variant by the exclusion of exon 29 coding for 19 amino acids in the extracellular loop connecting transmembrane domains IVS3 and IVS4. Like the adult splice variant CaV1.1a, the embryonic CaV1.1e variant functions as voltage sensor in excitation-contraction coupling, but unlike CaV1.1a it also conducts sizable calcium currents. Consequently, physiological or pharmacological modulation of calcium currents may have a greater impact in CaV1.1e expressing muscle cells. Here, we analyzed the effects of L-type current modulators on whole-cell current properties in dysgenic (CaV1.1-null) myotubes reconstituted with either CaV1.1a or CaV1.1e. Furthermore, we examined the physiological current modulation by interactions with the ryanodine receptor using a chimeric CaV1.1e construct in which the cytoplasmic II-III loop, essential for skeletal muscle excitation-contraction coupling, has been replaced with the corresponding but nonfunctional loop from the Musca channel. Whereas the equivalent substitution in CaV1.1a had abolished the calcium currents, substitution of the II-III loop in CaV1.1e did not signific...Continue Reading

References

Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B P Bean
May 8, 1995·Biochimica Et Biophysica Acta·W MelzerH C Lüttgau
Jun 1, 1996·The Journal of General Physiology·R T Dirksen, K G Beam
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M GrabnerK G Beam
Dec 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Antonios PantazisRiccardo Olcese

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Citations

Jun 9, 2015·Experimental Physiology·Diane LipscombeStephanie Schorge
Nov 30, 2016·The Journal of Physiology·Bernhard E Flucher, Petronel Tuluc
Nov 24, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christine P ShenSean M Wilson
Jan 13, 2018·The Journal of Biological Chemistry·Donald BeqollariRoger A Bannister
Oct 3, 2018·Communications Biology·Xaver KoenigBradley S Launikonis

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Methods Mentioned

BETA
transfection

Software Mentioned

pClamp
METAVUE

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