Physiological and Transcriptional Responses in Weaned Piglets Fed Diets with Varying Phosphorus and Calcium Levels

Nutrients
Christian GerlingerK Wimmers

Abstract

Phosphorus (P) is an important element of various metabolic and signalling processes, including bone metabolism and immune function. To elucidate the routes of P homeostasis and utilization, a five-week feeding study was conducted with weaned piglets receiving a diet with recommended amounts of P and Ca (M), or a diet with lower (L) or higher (H) P values and a constant Ca:P ratio. Routes of P utilization were deduced via bone characteristics (MicroCT), genome-wide transcriptomic profiles of peripheral blood mononuclear cells (PBMCs), and serum mineral levels. MicroCT revealed significantly lower bone mineral density, trabecular number, and mechanical fracture load in (L). Gene expression analyses showed transcripts of 276 and 115 annotated genes with higher or lower abundance in (H) than (L) that were related to basic cellular and metabolic processes as well as response to stimuli, developmental processes and immune system processes. This study shows the many molecular routes involved in P homeostasis that should be considered to improve endogenous mechanisms of P utilization.

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Citations

Mar 18, 2020·Journal of Animal Physiology and Animal Nutrition·Christian GerlingerPetra Wolf
Apr 23, 2020·International Journal of Molecular Sciences·Michael OsterKlaus Wimmers
Dec 2, 2020·Animals : an Open Access Journal From MDPI·Malgorzata HabichPawel Szczesny

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Methods Mentioned

BETA
X-Ray
PCR
dissection

Software Mentioned

PANTHER
SAS
Affymetrix GCOS
Expression Console (
R

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