Physiological and Transcriptomic Analysis of a Chronologically Long-Lived Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering

Molecular Biotechnology
Mevlüt ArslanZeynep Petek Çakar

Abstract

High-throughput aging studies with yeast as a model organism involve transposon-mutagenesis and yeast knockout collection, which have been pivotal strategies for understanding the complex cellular aging process. In this study, a chronologically long-lived Saccharomyces cerevisiae mutant was successfully obtained by using another high-throughput approach, evolutionary engineering, based on systematic selection in successive batch cultures under gradually increasing levels of caloric restriction. Detailed comparative physiological and transcriptomic analyses of the chronologically long-lived mutant and the reference strain revealed enhanced levels of respiratory metabolism, upregulation of genes related to carbohydrate metabolic processes, glycogen-trehalose pathways, stress response, and repression of protein synthesis-related genes in the long-lived mutant SRM11, already in the absence of caloric restriction. Interestingly, SRM11 had also significantly higher resistance to copper stress, and higher resistance to silver, ethanol, and 2-phenylethanol stresses than the reference strain. It also had lower ethanol production levels and an enhanced ethanol catabolism. To conclude, evolutionary engineering is another powerful high-thr...Continue Reading

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Citations

Nov 16, 2019·World Journal of Microbiology & Biotechnology·Yusuf SürmeliZeynep Petek Çakar
May 26, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Yasmin CherniLobna Elleuch
Aug 31, 2021·World Journal of Microbiology & Biotechnology·Alica NavratilovaMiroslava Pozgajova

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