Nov 6, 2018

Physiological and transcriptomic analysis of yellow leaf

BioRxiv : the Preprint Server for Biology
Shuzhen ZhangTiantian Lin

Abstract

As important deciduous tree, Populus deltoides Marsh possesses a high ornamental value for its leaves remaining yellow during the non-dormant period. However, little is known about the regulatory mechanism of leaf coloration in Populus deltoides Marsh. Thus, we analyzed physiological and transcriptional differences of yellow leaves (mutant) and green leaves (wild-type) of Populus deltoides Marsh. Physiological experiments showed that the contents of chlorophyll (Chl) and carotenoid are lower in mutant, the flavonoid content is not differed significantly between mutant and wild-type. Transcriptomic sequencing was further used to identify 153 differentially expressed genes (DEGs). Functional classifications based on Gene Ontology enrichment and Genomes enrichment analysis indicated that the DEGs were involved in Chl biosynthesis and flavonoid biosynthesis pathway. Among these, geranylgeranyl diphosphate (CHLP) genes associated with Chl biosynthesis showed down-regulation, while chlorophyllase (CLH) genes associated with Chl degradation were up-regulated in yellow leaves. The expression levels of these genes were further confirmed using quantitative real-time PCR (RT-qPCR). Furthermore, the measurement of the main precursors of Ch...Continue Reading

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Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Biochemical Pathway
Verloove-Vanhorick Brubakk Syndrome
Laboratory Procedures
Allergy Testing Poplar
Classification
poplar antigen
Carotenoids
Genome
Genes

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