Physiological consequences associated with overproduction of Mycobacterium tuberculosis FtsZ in mycobacterial hosts

Microbiology
Jaroslaw DziadekMalini Rajagopalan

Abstract

The ftsZ gene of Mycobacterium tuberculosis H37Rv has been characterized as the first step in determining the molecular events involved in the cell division process in mycobacteria. Western analysis revealed that intracellular levels of FtsZ are growth phase dependent in both M. tuberculosis and Mycobacterium smegmatis. Unregulated expression of M. tuberculosis ftsZ from constitutive hsp60 and dnaA promoters in M. tuberculosis hosts resulted in lethality whereas expression from only the hsp60 promoter was toxic in M. smegmatis hosts. Expression of ftsZ from the dnaA promoter in M. smegmatis resulted in approximately sixfold overproduction and the merodiploids exhibited slow growth, an increased tendency to clump and filament, and in some cases produced buds and branches. Many of the cells also contained abnormal and multiple septa. Expression of ftsZ from the chemically inducible acetamidase promoter in M. smegmatis hosts resulted in approximately 22-fold overproduction of FtsZ and produced filamentous cells, many of which lacked any visible septa. Visualization of the M. tuberculosis FtsZ tagged with green fluorescent protein in M. smegmatis by fluorescence microscopy revealed multiple fluorescent FtsZ foci, suggesting that st...Continue Reading

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Citations

Dec 7, 2011·Tuberculosis·Erin MaloneyMurty Madiraju
Nov 19, 2011·Tuberculosis·Indumathi S VadrevuMalini Rajagopalan
Dec 17, 2009·Tuberculosis·Manjot KiranMalini Rajagopalan
Dec 17, 2009·Tuberculosis·Erin MaloneyMalini Rajagopalan
Dec 17, 2009·Tuberculosis·Manjot KiranMalini Rajagopalan
May 24, 2007·Journal of Basic Microbiology·Ramanujam Srinivasan, Parthasarathi Ajitkumar
Jul 22, 2014·Clinical and Experimental Immunology·M A BartlomiejczykM Cedzynski
May 11, 2005·Biochemical and Biophysical Research Communications·Malini RajagopalanMurty V Madiraju
Apr 6, 2007·Biochemical and Biophysical Research Communications·Ramanujam SrinivasanParthasarathi Ajitkumar
Apr 28, 2006·Microbiological Research·Ramanujam SrinivasanParthasarathi Ajitkumar
Apr 25, 2006·FEMS Microbiology Letters·Malgorzata Korycka-MachalaJaroslaw Dziadek
Feb 27, 2016·Molecular Microbiology·Kirsten FamullaHeike Brötz-Oesterhelt
Sep 1, 2006·Molecular Microbiology·Ashwini ChauhanMalini Rajagopalan
Jun 16, 2018·The Journal of Biological Chemistry·Preeti JainVinay Kumar Nandicoori
Jan 11, 2012·Antimicrobial Agents and Chemotherapy·Santiago Ramón-GarcíaJosé A Aínsa
Feb 7, 2016·Applied and Environmental Microbiology·Alex I KannoJohnjoe McFadden
May 9, 2006·Molecular Microbiology·Rut Carballido-López
Dec 14, 2002·Microbiology·Rebecca GreendykeMurty V V S Madiraju
Jun 5, 2003·Microbiology·Jaroslaw DziadekMalini Rajagopalan
Jul 9, 2002·The Journal of Biological Chemistry·Pratik DattaJoyoti Basu
Jun 6, 2007·Antimicrobial Agents and Chemotherapy·Malgorzata Korycka-MachalaJarosław Dziadek
Feb 18, 2006·Journal of Bacteriology·Ashwini ChauhanMalini Rajagopalan
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Harold P EricksonMasaki Osawa
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Erik C Hett, Eric J Rubin
Oct 28, 2006·The Journal of Biological Chemistry·Meghna Thakur, Pradip K Chakraborti
May 23, 2012·The Journal of Biological Chemistry·Renata PlocinskaMalini Rajagopalan

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