PMID: 9421969Jan 9, 1998Paper

Physiological doses of ultraviolet irradiation induce DNA strand breaks in cultured human melanocytes, as detected by means of an immunochemical assay

Photochemistry and Photobiology
E WenczlA A Schothorst

Abstract

An immunochemical assay, i.e. sandwich enzyme-linked immunosorbent assay, has been modified to detect UV-induced damage in cellular DNA of monolayer-grown human melanocytes. The method is based on the binding of a monoclonal antibody to single-stranded DNA. The melanocytes derived from human foreskin of skin type II individuals were suspended and exposed to UVA, UVB, solar-simulated light or gamma-rays. Following physiological doses of UVA, UVB or solar-simulated light, a dose-related DNA unwinding comprising a considerable number of single-strand breaks (ssb) was observed. No correlation was found between different seeded cell densities or different culturing periods and the UVA sensitivity of the cells. After UVA irradiation, 0.07 ssb/10(10) Da/kJ/m2 were detected and after UVB irradiation 1.9 ssb/10(10) Da/kJ/m2 were seen. One minimal erythema dose of solar-simulated light induced 2.25 ssb/10(10) Da. Our results from melanocytes expressed in ssb/Da DNA are comparable and have the same sensitivity toward UVA as well as toward UVB as nonpigmented skin cells. As low doses of UVA have already been shown to induce detectable numbers of ssb, this assay is of great interest for further investigations about the photoprotecting and/o...Continue Reading

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Citations

May 18, 2010·Analytical and Bioanalytical Chemistry·Min Jung KimJoon Myong Song
Jun 27, 2001·Free Radical Biology & Medicine·S D'AngeloP Galletti
May 27, 2006·Journal of Toxicology and Environmental Health. Part a·Anna Brozyna, Barbara W Chwirot
Feb 6, 2008·Photochemistry and Photobiology·Nityanand Maddodi, Vijayasaradhi Setaluri
Dec 17, 2008·Photochemistry and Photobiology·Cestmír CejkaJitka Cejková
Sep 3, 2010·International Journal of Radiation Biology·Alena Svobodová, Jitka Vostálová
Oct 9, 1998·The Journal of Investigative Dermatology·E WenczlA A Schothorst
Aug 22, 2000·The Journal of Investigative Dermatology·G EmriA A Schothorst
Nov 4, 2000·Dermatologic Clinics·H V DeBuysS R Pinnell
Feb 24, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·S R PinnellM Levine
Jan 11, 2003·Journal of the American Academy of Dermatology·Sheldon R Pinnell

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