Physiological effect of the alterations of plasmids derived from Neisseria gonorrhoeae

Infection
P DomenicoB A Cunha

Abstract

Transfer of plasmid DNA from penicillinase-producing Neisseria gonorrhoeae to Escherichia coli produced relatively small colonies that became pigmented in the presence of the dye, congo red. Mutations to large, non-pigmented colonies were frequent, and were the result of a variety of alterations in these plasmids. Miniplasmid formation was the major alteration that led to non-pigmented colonies. However, deletions in any of three putative replication regions resulted in non-pigmented colonies. These altered plasmids mediated significantly less beta-lactamase production than that of the parent plasmid. A new origin of replication was discovered and mapped within the miniplasmid. This origin was responsible for: 1) high plasmid copy number, 2) stability of inheritance, and 3) the majority of beta-lactamase produced in culture. The evolution of gonococcal plasmids may be followed in E. coli, since less defective plasmids produce nonpigmented colonies on congo red agar medium.

References

Dec 1, 1977·Journal of Bacteriology·D P TaylorR H Rownd
Feb 1, 1985·The Journal of Antimicrobial Chemotherapy·J D Van EmbdenB Van Klingeren
Jun 1, 1986·Antimicrobial Agents and Chemotherapy·A GoubyM Ramuz
Apr 1, 1972·Antimicrobial Agents and Chemotherapy·C H O'CallaghanA H Shingler

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Citations

Aug 20, 2005·Nature Reviews. Immunology·Thomas DeckerSilvia Stockinger

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