Abstract
The yeast Saccharomyces cerevisiae has been studied during cultivation with a naturally occurring antimycotic azasterol. At very low concentrations (1.0 to 10.0 ng/ml), where growth retardation is not observed, an unusual sterol, ergosta-8,14-dien-3beta-ol, accumulates in high concentrations. Upon removal of the azasterol from the culture, the 8,14-diene is converted to ergosterol. Much smaller amounts of another 8,14-sterol, but with an additional unsaturation, have also been observed. Total sterol accumulation is higher in cultures containing subinhibitory levels of the antimycotic agent than the amounts of normal sterol accumulation in control cultures. With between 10 and 100 ng of azasterol per ml a transitory cessation of growth is observed from which the culture is able to recover. At much higher concentrations growth inhibition and even cell lysis results. Competitive inhibition of sterol 24(28)methylene reductase is demonstrated.
References
Mar 1, 1977·Journal of Bacteriology·W D Neal, L W Parks
Jul 1, 1976·Canadian Journal of Biochemistry·L AvruchA C Oehlschlager
Dec 1, 1976·Journal of Bacteriology·R B BaileyL W Parks
Oct 1, 1976·Lipids·J J KabaraD P Catsoulacos
Feb 1, 1975·The Journal of Antibiotics·L D BoeckD N Thomas
Nov 1, 1975·Journal of Bacteriology·R B Bailey, L W Parks
Dec 22, 1973·Biochimica Et Biophysica Acta·B de KruyffL L van Deenen
Jan 1, 1971·The Biochemical Journal·I A WatkinsonM Akhtar
Dec 1, 1974·The Journal of Antibiotics·J W ChamberlinJ L Occolowitz
Feb 26, 1974·Biochimica Et Biophysica Acta·B de Kruijff, R A Demel
Nov 1, 1969·The Biochemical Journal·M AkhtarI A Watkinson
Citations
Oct 1, 1978·Lipids·L W ParksS Hough
Mar 1, 1982·Lipids·L W ParksJ R Ramp
Mar 1, 1995·Lipids·L W ParksJ H Crowley
Dec 1, 1985·Sabouraudia·A Polak-WyssG Oesterhelt
Jul 1, 1979·Antimicrobial Agents and Chemotherapy·C P WoloshukS R Dutky
Nov 1, 1980·Antimicrobial Agents and Chemotherapy·R J Rodriguez, L W Parks
Aug 1, 1981·Antimicrobial Agents and Chemotherapy·R J Rodriguez, L W Parks
Apr 1, 1983·Antimicrobial Agents and Chemotherapy·F R TaylorL W Parks
Jun 1, 1985·Antimicrobial Agents and Chemotherapy·D P KasbekarE R Katz
Aug 1, 1991·Antimicrobial Agents and Chemotherapy·R T Lorenz, L W Parks
Jan 1, 1978·CRC Critical Reviews in Microbiology·L W Parks
Dec 22, 1978·Biochimica Et Biophysica Acta·C K Bottema, L W Parks
Jun 23, 1978·Biochimica Et Biophysica Acta·H D PierceA C Oehlschlager
Oct 1, 1990·Applied and Environmental Microbiology·H R Burmeister, R F Vesonder
Nov 1, 1978·Journal of Bacteriology·F R Taylor, L W Parks