Physiological exposure to melatonin supersensitizes the cyclic adenosine 3',5'-monophosphate-dependent signal transduction cascade in Chinese hamster ovary cells expressing the human mt1 melatonin receptor

Endocrinology
P A Witt-EnderbyM L Dubocovich

Abstract

Here, we report the effects of short exposure to melatonin on the human mt1 (h mt1) melatonin receptor-mediated signaling in Chinese hamster ovary (CHO) cells, and the consequences of an exposure that resembles the physiological pattern of melatonin release on cAMP-mediated signal transduction. Short exposure (10 min) of h mt1 melatonin receptors to melatonin (400 pM) inhibited forskolin-stimulated cAMP formation, cAMP-dependent protein kinase activity, and phosphorylation of the cAMP response element-binding protein. However, treatment of mt1-CHO cells with melatonin in a manner that closely mimics the in vivo activation of melatonin receptors (i.e. 400 pM melatonin for 8 h to mimic darkness) resulted in a supersensitization of the cAMP-dependent signal transduction cascade during the period of withdrawal (i.e. 16 h without melatonin to mimic the light cycle of a diurnal photoperiod). During the period of withdrawal, forskolin induced a time-dependent (1-16 h) increase in cAMP formation (approximately 200% of control cells). This effect of melatonin was dependent on the presence of the h mt1 melatonin receptor, as no potentiation of forskolin-induced cAMP formation was observed in CHO cells transfected only with the neomycin r...Continue Reading

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Citations

Nov 6, 2014·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Sameer Gupta, Chandana Haldar
Dec 28, 2016·Chemical Research in Toxicology·Marina Popovska-GorevskiRajendram V Rajnarayanan
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Mar 29, 2013·International Journal of Molecular Sciences·Elmar PeschkeEckhard Mühlbauer
Mar 24, 2018·Journal of Pineal Research·Amanda LochnerBarbara Huisamen
Oct 12, 2018·Archives of Endocrinology and Metabolism·Fernanda Gaspar do Amaral, José Cipolla-Neto
Dec 18, 2014·Reproduction, Fertility, and Development·Marta Gonzalez-ArtoAdriana Casao
May 22, 2008·British Journal of Pharmacology·R JockersP Delagrange
Jul 9, 2010·Journal of Pineal Research·Lulu MaoSteven M Hill
Sep 15, 2018·Endocrine Reviews·José Cipolla-Neto, Fernanda Gaspar do Amaral
Oct 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·J A Cebrián-PérezT Muiño-Blanco
Jul 21, 2001·Molecular Endocrinology·C S NelsonC N Allen
Mar 25, 2017·Biology of Reproduction·Alfredo Ramírez-RevecoIlona I Concha
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Mar 8, 2003·Journal of Neuroendocrinology·P BarrettP J Morgan
Apr 24, 2015·Journal of Pineal Research·Elmar PeschkeEckhard Mühlbauer
Apr 2, 2014·Frontiers in Neuroendocrinology·Sarah A LaredoBrian C Trainor
Aug 7, 2021·Hormone Molecular Biology and Clinical Investigation·Ming Guan NgSoi Moi Chye
Nov 8, 2001·Science's STKE : Signal Transduction Knowledge Environment·M I Masana, M L Dubocovich

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