PMID: 3492Mar 1, 1976

Physiological factors affecting transformation of Azotobacter vinelandii

Journal of Bacteriology
W J Page, H L Sadoff

Abstract

Cells of Azotobacter vinelandii (ATCC 12837) can be transformed by exogenous deoxyribonucleic acid towards the end of exponential growth. Transformation occurs at very low frequencies when the deoxyribonucleic acid is purified or when the transformation is carried out in liquid medium. Optimal transformation occurs on plates of Burk nitrogen-free glucose medium containing either high phosphate (10 mM) or low calcium (0 to 0.29 mM) content. Higher levels of calcium are inhibitory, whereas magnesium ions are essential for transformation and growth. Extracellular polymer and capsule are increasingly inhibitory to transformation and are most abundant when the calcium content of the medium is high. Transformation is optimal at pH 7.0 to 7.1 and at 30 C, conditions which also coincide with minimal extracellular polymer production. Nonencapsulated strains are excellent transformation recipients. Glycine-induced pleomorphism reduces the transformation frequency and the degree of inhibition is dependent on the phosphate concentration of the medium. Rifampin resistance and shifts from adenine, hypoxanthine, uracil, and nitrogenase auxotrophy to prototrophy can be achieved. Although single marker transfer is always greater than double mar...Continue Reading

Citations

Nov 1, 1979·Biological Reviews of the Cambridge Philosophical Society·P J Piggot
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Jesse D Noar, José M Bruno-Bárcena
Nov 1, 2016·Applied Microbiology and Biotechnology·Carlos Leonel Ahumada-ManuelCinthia Núñez
May 11, 2010·Applied and Environmental Microbiology·Nanxi LuThanh H Nguyen

Related Concepts

Adenine
Azotobacter
Calcium
Antibiotic Resistance, Microbial
Anhydrous Dextrose
Hydrogen-Ion Concentration
Hypoxanthines
Magnesium
Vanadium Nitrogenase
Orthophosphate

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