Physiological factors involved in the transformation of Mycobacterium smegmatis.

Journal of Bacteriology
M V Norgard, T Imaeda

Abstract

Transfer of streptomycin resistance and changes from methionine and leucine auxotrophy to prototrophy were achieved in Mycobacterium smegmatis by transformation. Recipient cells were more resistant to mitomycin C and methyl methlanesulfonate treatments than were wild-type cells. A high level of calcium ions was essential for transformation, especially during DNA adsorption, whereas the presence of magnesium ions and the exposure of recipient cells to mild doses of UV light enhanced recombination frequencies. Transformants were not isolated when recipient cell-DNA mixtures were first treated with deoxyribonuclease. Recipient cells at various stages of growth showed similar transformabilities. Transformation was successful only when recipient cells were incubated on rich agar medium after mixture with DNA. Exposure of recipient cells to Pronase before treatment with donor DNA did not affect transformation, suggesting the absence of a protein competence factor. Throughout the present experiments, cotransformation frequencies were very low and unselected-marker segregation patterns were independent, indicating that the methionine, leucine, and streptomycin markers are not closely linked in M. smegmatis.

Citations

Sep 1, 2014·Genome Biology and Evolution·Tatum D Mortimer, Caitlin S Pepperell
Jan 1, 1988·Folia Microbiologica·J KonícekM Slosárek
Dec 1, 1983·Archives of Microbiology·S S Karnik, K P Gopinathan
Feb 19, 2002·Molecular Microbiology·Apoorva BhattTobias Kieser
Jul 6, 2000·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·F BertollaP Simonet
Nov 1, 1980·Journal of Bacteriology·M V Norgard, T Imaeda
Jan 4, 1994·Biochimica Et Biophysica Acta·R PalmenK J Hellingwerf
Sep 1, 1994·Microbiological Reviews·M G Lorenz, W Wackernagel
Nov 13, 2007·Research in Microbiology·Ola JohnsborgLeiv Sigve Håvarstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.