Physiological levels of glutamine prevent morphine-induced preconditioning in the isolated rat heart

European Journal of Pharmacology
André HeinenNina C Weber

Abstract

Morphine induces cardioprotection against ischaemia-reperfusion injury. While aiming to investigate the underlying signal transduction cascade of morphine preconditioning in isolated Langendorff-perfused rat hearts, the expected cardioprotection was not detectable. Thus, we investigated the influence of different preconditioning protocols and substrate conditions on cardioprotection in this experimental model. Isolated rat hearts underwent 35 min global ischaemia followed by 60 min reperfusion. Morphine PC was initiated by 3 cycles of 5 min 1 muM morphine with either 5 min washout [3PC5 (5)] or 15 min washout [3PC5 (15)] before ischaemia; by 15 min morphine with 15 min washout before ischaemia [PC15 (15)]; or by 15 min 10 muM morphine with 15 min washout [PC15 (15)-10 microM]. Ischaemic preconditioning was initiated by 3 cycles of 3 min ischaemia; in another group, hearts received 1 muM morphine continuously for 10 min before ischaemia until the end of reperfusion [continued morphine]. To investigate the effects of glutamine, two groups received a glutamine-free perfusate: a control group, and a morphine preconditioning group [3PC5 (15)]. Ischaemic preconditioning reduced infarct size by 75%, and continued morphine by 46% compa...Continue Reading

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Citations

Oct 2, 2009·Peptides·Richard J Bodnar
Jun 12, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andrew J Murray, Hugh E Montgomery
Apr 30, 2017·Cardiovascular Research·Anne VincentStéphanie Barrère-Lemaire

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