Physiological responses to cold stress in the gills of discus fish (Symphysodon aequifasciatus) revealed by conventional biochemical assays and GC-TOF-MS metabolomics

The Science of the Total Environment
Bin WenJian-Zhong Gao

Abstract

Discus fish (Symphysodon aequifasciatus) is a cichlid that is among the most popular fish for warm-water aquaria and also frequently used as the model animal for environmental science. However, little is known about the responses of S. aequifasciatus to low temperatures caused by environmental variation. Here, by using conventional biochemical assays and gas chromatography time-of-flight mass spectrometry metabolomics, we investigated the physiological responses of S. aequifasciatus gills exposed for 30 days to two temperature regimes: 28 °C and 20 °C. Low temperature resulted in elevated production of reactive oxygen species but not increased malondialdehyde. This might be partially related to protective responses in the antioxidant system, revealed by increased activities of superoxide dismutase and glutathione peroxidase, and level of reduced glutathione (GSH), compensating for the depletion of catalase activity. A total of 35 metabolites were identified as potential biomarkers of cold stress, showing the most influenced pathways including starch and sucrose metabolism, pentose phosphate pathway, glycerolipid metabolism, sphingolipid metabolism, glutathione metabolism, and arginine and proline metabolism. Moreover, the activ...Continue Reading

Citations

Mar 4, 2020·Journal of Thermal Biology·Carla BacchettaJimena Cazenave
Dec 17, 2020·Animals : an Open Access Journal From MDPI·Minmeng ZhaoDaoqing Gong
Apr 12, 2020·Fish & Shellfish Immunology·Shuangshuang YangChenhao Yuan
Aug 22, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Nina GouBinxin Zhang

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