Physiological screening for target site insensitivity and localization of Na(+)/K(+)-ATPase in cardenolide-adapted Lepidoptera

Journal of Insect Physiology
Georg PetschenkaSusanne Dobler

Abstract

Cardenolides are toxic plant compounds which specifically inhibit Na(+)/K(+)-ATPase, an animal enzyme which is essential for many physiological processes, such as the generation of action potentials. Several adapted insects feeding on cardenolide-containing plants sequester these toxins for their own defence. Some of these insects were shown to possess Na(+)/K(+)-ATPases with a reduced sensitivity towards cardenolides (target site insensitivity). In the present study we screened five species of arctiid moths feeding on cardenolide-containing plants for target site insensitivity towards cardenolides using an in vitro enzyme assay. The derived dose response curves of the respective Na(+)/K(+)-ATPases were compared to the insensitive Na(+)/K(+)-ATPase of the monarch butterfly (Danaus plexippus). Na(+)/K(+)-ATPases of all arctiid species tested were highly sensitive to ouabain, a water-soluble cardenolide which is most widely used in laboratory studies. Nevertheless, we detected substantial amounts of cardenolides in the haemolymph of two of the arctiid species. In caterpillars of the sequestering arctiid Empyreuma pugione and of D. plexippus we localized Na(+)/K(+)-ATPase by immunohistochemistry and western blot (in D. plexippus)....Continue Reading

References

May 15, 1973·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M RothschildT Reichstein
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·L P BrowerJ M Corvino
Jun 1, 1970·Journal of Insect Physiology·M RothschildT Reichstein
Jan 1, 1994·Journal of Neurobiology·C L MurrayC E Morris
Jun 27, 2000·Cell and Tissue Research·J C YasuharaK Takeyasu
May 29, 2003·Journal of Insect Physiology·M B.A. DjamgozA M. Emery
Sep 2, 2003·Molecular Biology and Evolution·Estelle Labeyrie, Susanne Dobler
Sep 16, 2003·The Journal of Biological Chemistry·Li Yan QiuJan Joep H H M De Pont
Feb 1, 1965·Journal of Cellular Physiology·J A HASKELLW R HARVEY
May 1, 1965·The Journal of Physiology·J A PARSONS
Mar 22, 2005·Toxicon : Official Journal of the International Society on Toxinology·Dietrich MebsMichael Schneider
Dec 25, 2010·Journal of Structural Biology·Laure YatimeNatalya U Fedosova

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Citations

Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Susanne DoblerAnurag A Agrawal
Mar 14, 2013·Journal of Chemical Ecology·Jennifer Sorensen ForbeyWilliam J Foley
Apr 29, 2014·Journal of Agricultural and Food Chemistry·Rebecca E IrwinDale R Gardner
Oct 21, 2016·Evolution; International Journal of Organic Evolution·Safaa Dalla, Susanne Dobler
Nov 6, 2015·Proceedings. Biological Sciences·Georg Petschenka, Anurag A Agrawal
Jan 27, 2017·PloS One·Yallappa Rajashekar, Thimmappa Shivanandappa
Nov 18, 2016·Proceedings. Biological Sciences·Shabnam MohammadiAlan H Savitzky
Dec 11, 2017·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shabnam MohammadiAlan H Savitzky
Dec 25, 2016·Insect Biochemistry and Molecular Biology·Simon C GroenNoah K Whiteman

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