PMID: 2123855Dec 1, 1990Paper

Physiological studies on regulation of glycerol utilization by the phosphoenolpyruvate:sugar phosphotransferase system in Enterococcus faecalis

Journal of Bacteriology
A H RomanoJ Reizer

Abstract

In vitro studies with purified glycerol kinase from Enterococcus faecalis have established that this enzyme is activated by phosphorylation of a histidyl residue in the protein, catalyzed by the phosphoenolpyruvate-dependent phosphotransferase system (PTS), but the physiological significance of this observation is not known. In the present study, the regulation of glycerol uptake was examined in a wild-type strain of E. faecalis as well as in tight and leaky ptsI mutants, altered with respect to their levels of enzyme I of the PTS. Glycerol kinase was shown to be weakly repressible by lactose and strongly repressible by glucose in the wild-type strain. Greatly reduced levels of glycerol kinase activity were also observed in the ptsI mutants. Uptake of glycerol into intact wild-type and mutant cells paralleled the glycerol kinase activities in extracts. Glycerol uptake in the leaky ptsI mutant was hypersensitive to inhibition by low concentrations of 2-deoxyglucose or glucose even though the rates and extent of 2-deoxyglucose uptake were greatly reduced. These observations provide strong support for the involvement of reversible PTS-mediated phosphorylation of glycerol kinase in the regulation of glycerol uptake in response to t...Continue Reading

References

Mar 1, 1974·Journal of Bacteriology·F M Harold, E Levin
Dec 1, 1965·Journal of Molecular Biology·S I Hayashi, E C Lin
Feb 1, 1983·Journal of Bacteriology·S S Dills, S Seno
Nov 22, 1982·Biochimica Et Biophysica Acta·A M Slee, J M Tanzer
Sep 1, 1958·Journal of Bacteriology·M L MORSE, M L ALIRE

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Citations

Jan 1, 1997·Folia Microbiologica·J DeutscherV Dossonnet
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Feb 1, 1996·Microbiology·Milton H SaierJing-Jing Ye
Nov 1, 1995·Journal of Bacteriology·E WagnerR Brückner
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson

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