Physiological variations in ovine cerebrovascular calcium sensitivity

The American Journal of Physiology
S E AkopovWilliam J Pearce

Abstract

Cerebrovascular reactivity to biogenic amines varies in relation to both arterial diameter and age. The present study examines the hypothesis that these patterns of reactivity are secondary to corresponding variations in the Ca2+ sensitivity of the contractile proteins. To test this hypothesis, we permeabilized segments of common carotid (Com), basilar, main branch middle cerebral, and second-branch middle cerebral (MCA-B) arteries from nonpregnant adult and near-term fetal sheep using beta-escin. Permeabilization methods were carefully validated and adjusted for each artery type. Baseline myofilament Ca2+ sensitivity in both adults and fetuses increased significantly from the Com to the MCA-B and was generally higher in fetuses than in adults. Serotonin dose dependently increased Ca2+ sensitivity via a G protein-dependent mechanism in all arteries. The magnitudes of this effect did not vary among artery types but were significantly greater in fetal than in adult arteries. This effect of serotonin was mimicked by guanosine 5'-O-(3-thiotriphosphate), a nonhydrolyzable analog of guanosine 5'-triphosphate, and its effects were also much greater in fetal than in adult arteries. We conclude that patterns of cerebrovascular reactivit...Continue Reading

Citations

Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D M AngelesW J Pearce
Feb 24, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D M AngelesW J Pearce
Apr 12, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Mike T LinLawrence D Longo
Feb 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Mike T LinDavid A Hessinger
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Cristina Camello-AlmarazMaría J Pozo
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S M NauliW J Pearce
Jul 15, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Mike T LinLawrence D Longo
Nov 8, 2001·American Journal of Physiology. Cell Physiology·S M NauliW J Pearce
Jun 8, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shelton M CharlesWilliam J Pearce
Mar 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Renan J SandovalWilliam J Pearce
Jul 31, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Renan J SandovalWilliam J Pearce
Jul 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Abu AhmedJonathan H Jaggar
Jul 20, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Shelton M CharlesWilliam J Pearce
Feb 28, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Daliao XiaoLubo Zhang
Oct 7, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Elisha R InjetiWilliam J Pearce
Sep 11, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James M WilliamsWilliam J Pearce
Jul 29, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dane W SorensenWilliam J Pearce

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.