Phytase production by Sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread

Journal of Applied Microbiology
Bijender Singh, T Satyanarayana

Abstract

Phytase production by Sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread. The production of phytase by a thermophilic mould S. thermophile was investigated using free and immobilized conidiospores in cane molasses medium in shake flasks, and stirred tank and air-lift fermenters. Among surfactants tested, Tweens (Tween-20, 40 and 80) and sodium oleate increased phytase accumulation, whereas SDS and Triton X-100 inhibited the enzyme production. The mould produced phytase optimally at a(w) 0.95, and it declined sharply below this a(w) value. The enzyme production was comparable in air-lift and stirred tank reactors with a marked reduction in fermentation time. Among the matrices tried, Ca-alginate was the best for conidiospore immobilization, and fungus secreted sustained levels of enzyme titres over five cycles. The phytic acid in the dough was efficiently hydrolysed by the enzyme accompanied by the liberation of soluble phosphate in the bread. The phytase production by S. thermophile was enhanced in the presence of Tween-80 in cane molasses medium. A peak in enzyme production was attained in 48 h in the fermenter when compared with that of 96 h in shake flasks...Continue Reading

References

Jul 19, 2000·Enzyme and Microbial Technology·A BlandinoD Cantero
Aug 9, 2001·Journal of Industrial Microbiology & Biotechnology·G Díaz-GodínezG Viniegra-González
Nov 21, 2001·Journal of Agricultural and Food Chemistry·M HarosC Benedito
Apr 16, 2003·Critical Reviews in Biotechnology·Ashima Vohra, T Satyanarayana
Oct 11, 2003·Biotechnology Advances·J K Park, H N Chang
Aug 12, 2004·Applied Biochemistry and Biotechnology·K Madhavan NampoothiriAshok Pandey

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Citations

Apr 1, 2009·Applied Biochemistry and Biotechnology·Bijender Singh, T Satyanarayana
Jan 19, 2016·Critical Reviews in Microbiology·Bijender SinghTulasi Satyanarayana
Nov 20, 2015·Bioprocess and Biosystems Engineering·Anju Bala, Bijender Singh
Dec 5, 2008·Bioresource Technology·Bijender Singh, T Satyanarayana
May 10, 2015·Applied Biochemistry and Biotechnology·Swati Joshi, Tulasi Satyanarayana
Mar 19, 2016·Journal of the Science of Food and Agriculture·Karim Rocky-SalimiMaryam Mousivand
Dec 18, 2013·Applied Microbiology and Biotechnology·Mohamed Ali BorgiMoez Rhimi
Aug 19, 2014·Journal of Animal Physiology and Animal Nutrition·B Singh, T Satyanarayana

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