Phytaspase, a relocalisable cell death promoting plant protease with caspase specificity.

The EMBO Journal
Nina V ChichkovaMichael E Taliansky

Abstract

Caspases are cysteine-dependent proteases and are important components of animal apoptosis. They introduce specific breaks after aspartate residues in a number of cellular proteins mediating programmed cell death (PCD). Plants encode only distant homologues of caspases, the metacaspases that are involved in PCD, but do not possess caspase-specific proteolytic activity. Nevertheless, plants do display caspase-like activities indicating that enzymes structurally distinct from classical caspases may operate as caspase-like proteases. Here, we report the identification and characterisation of a novel PCD-related subtilisin-like protease from tobacco and rice named phytaspase (plant aspartate-specific protease) that possesses caspase specificity distinct from that of other known caspase-like proteases. We provide evidence that phytaspase is synthesised as a proenzyme, which is autocatalytically processed to generate the mature enzyme. Overexpression and silencing of the phytaspase gene showed that phytaspase is essential for PCD-related responses to tobacco mosaic virus and abiotic stresses. Phytaspase is constitutively secreted into the apoplast before PCD, but unexpectedly is re-imported into the cell during PCD providing insights...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C E ShurvintonW Ream
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Feb 26, 1999·Nucleic Acids Research·M MatzA Chenchik
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·L Gómez-GómezT Boller
Jul 10, 1999·The Journal of Biological Chemistry·B B Wolf, D R Green
Dec 1, 1999·Cell Death and Differentiation·T S ZhengR A Flavell
Dec 1, 1999·Cell Death and Differentiation·P G EkertD L Vaux
Mar 16, 2000·Plant Science : an International Journal of Experimental Plant Biology·P MásV Pallás
Jun 1, 2000·Molecular Plant-microbe Interactions : MPMI·G Hansen
Feb 24, 2001·Plant Molecular Biology·M C Heath
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M B DickmanR French
May 23, 2002·Biochimie·Yoshihide Tsujimoto, Shigeomi Shimizu
Nov 13, 2002·Plant Physiology·Andreas NebenführDavid G Robinson
Jan 1, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Frank A Hoeberichts, Ernst J Woltering
Mar 15, 2003·The Journal of Biological Chemistry·Moonil KimHyun-Sook Pai
May 20, 2003·The Plant Journal : for Cell and Molecular Biology·Christophe LacommeIngo Hein
Dec 4, 2003·Current Opinion in Cell Biology·Kelly M Boatright, Guy S Salvesen
Dec 9, 2003·The Plant Cell·Nina V ChichkovaAndrey B Vartapetian
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Jean T. Greenberg
Jul 1, 2004·The Plant Journal : for Cell and Molecular Biology·Michael TalianskyHugh Barker
Aug 7, 2004·Science·Noriyuki HatsugaiIkuko Hara-Nishimura
Nov 9, 2004·Current Biology : CB·Enrique RojoNatasha V Raikhel
Jul 27, 2005·The Journal of Biological Chemistry·Miwa KuroyanagiIkuko Hara-Nishimura
Sep 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Peter V BozhkovAndrei Smertenko
Oct 10, 2006·Journal of Molecular Biology·Dominique VercammenFrank Van Breusegem
Mar 21, 2007·Plant Cell Reports·Brian ReavyMichael E Taliansky
Aug 19, 2007·The Plant Journal : for Cell and Molecular Biology·Eleanor M GilroyPaul R J Birch
Aug 19, 2007·Current Opinion in Plant Biology·Diane C Bassham
Nov 10, 2007·Nucleic Acids Research·Neil D RawlingsAlan J Barrett
Nov 14, 2007·The Journal of Biological Chemistry·Rui HePatrick Gallois
Feb 15, 2008·Journal of Experimental Botany·Laurent BonneauPatrick Gallois
Feb 14, 2009·Journal of Experimental Botany·Matthias Josef AffenzellerUrsula Lütz-Meindl

❮ Previous
Next ❯

Citations

Apr 16, 2011·Cell Death and Differentiation·W G van DoornP V Bozhkov
May 7, 2011·Cell Death and Differentiation·A B VartapetianT J Wolpert
Jul 12, 2011·Cell Death and Differentiation·P V Bozhkov, E Lam
Jan 4, 2012·Journal of Experimental Botany·Benjamin BollhönerHannele Tuominen
May 15, 2013·Journal of Experimental Botany·Israel JuliánManuel Martinez
Mar 29, 2012·The Plant Cell·Karina van der LindeGunther Doehlemann
Sep 2, 1985·European Journal of Biochemistry·E HollerH Simek
Nov 26, 2010·Science·Nuria S CollPetra Epple
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·A S FomichevaA B Vartapetian
Aug 7, 2013·Annual Review of Phytopathology·Martin B Dickman, Robert Fluhr
Jul 3, 2013·PLoS Pathogens·Vicente RamírezPablo Vera
Mar 14, 2014·Journal of Experimental Botany·Andrei Smertenko, Peter V Bozhkov
Sep 26, 2015·International Journal of Molecular Sciences·Miklós PogányZoltán Bozsó
Nov 7, 2012·Plant Physiology and Biochemistry : PPB·María Belén FernándezMaría Gabriela Guevara
Oct 23, 2012·Trends in Plant Science·Junhui Wang, Kenneth W Bayles
Dec 10, 2015·The FEBS Journal·Guy S SalvesenNuria S Coll
Feb 16, 2016·Biochemistry. Biokhimii︠a︡·A A Zamyatnin
Jun 12, 2012·The Plant Journal : for Cell and Molecular Biology·Jia-Jia HanXin-Qiang He
Jan 17, 2012·Physiologia Plantarum·Irma N RobertsChristiane Funk
Apr 9, 2013·Environmental Microbiology Reports·Mansha Seth-PasrichaKay D Bidle
Oct 20, 2011·Physiologia Plantarum·Liana TsiatsianiFrank Van Breusegem
Jun 4, 2014·The New Phytologist·Daniela SueldoRenier A L van der Hoorn
Sep 23, 2011·Biochemical Society Transactions·Derek Wilkinson, Mark Ramsdale
Apr 29, 2015·Frontiers in Plant Science·Noriyuki HatsugaiIkuko Hara-Nishimura
Mar 15, 2015·The International Journal of Biochemistry & Cell Biology·Lyudmila V SavvateevaGjumrakch Aliev
Jul 5, 2013·Environmental and Experimental Botany·Rajesh Kumar TewariIngeborg Lang
Dec 9, 2014·Plant Physiology and Biochemistry : PPB·María Belén FernándezMaría Gabriela Guevara
Jan 8, 2015·Frontiers in Plant Science·Andreia FigueiredoMónica Sebastiana
Oct 28, 2014·Frontiers in Plant Science·Volodymyr Radchuk, Ljudmilla Borisjuk
Sep 25, 2014·Annual Review of Marine Science·Kay D Bidle
Apr 30, 2016·Plant Signaling & Behavior·Yurong Li, Marty Dickman
Jun 5, 2016·Journal of Experimental Botany·Michael MeyerAndreas Schaller
Jul 13, 2016·Current Biology : CB·Kay D Bidle

❮ Previous
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