Apr 1, 1989

Phytic acid-enhanced metal ion exchange reactions: the effect on carboxypeptidase A

Journal of Inorganic Biochemistry
C J Martin, W J Evans

Abstract

On the basis of the known interaction of phytic acid to form soluble or insoluble complexes with cations, the effect of this naturally occurring polydentate ligand on carboxypeptidase A, a zinc-containing metalloenzyme, and its Co(II)-substituted derivative, has been studied. Under conditions of rigorous exclusion of adventitious metal ions, phytate showed no inhibitory effect. However, the addition of Cu(II) ions to form soluble phytate-Cu(II) complexes at pH 7.2 and 25 degrees C caused more than a 95% decrease in activity. The Cd(II) ion was nearly as effective but other ions showed only a small or no effect. In the absence of phytate, incubation of the enzyme with Cu(II) or Cd(II) at the same concentration produced only about a 25% reduction in activity. The decrease in activity followed first-order kinetics, and the rate constant was the same (1.2 x 10(-4) sec-1) as seen upon incubation with EDTA. However, in contrast to that observed upon incubation of the enzyme with phytate and Cu(II), exposure to EDTA produced a complete loss in activity which could be regained by addition of Zn(II) to the assay solution. In the former case, not only was there residual activity left after incubation at pH 7.2 for 24 hrs at 25 degrees C,...Continue Reading

Mentioned in this Paper

CPA1
Carboxypeptidase A
Carboxypeptidase A5
Apoenzymes
Complex (molecular entity)
Carboxypeptidase A Activity
Plasma Protein Binding Capacity
Copper
Soluble
Edetic Acid, Sodium Salt

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