Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra

Journal of Analytical Methods in Chemistry
Jing ZhenQingli Wu

Abstract

A method was developed for identification and quantification of polyphenols in the leaves of Ximenia caffra using HPLC/UV/MS. Based on analyzing the MS and UV data and in comparison to the authentic standards, a total of 10 polyphenols were identified and quantified, including gallic acid, catechin, quercetin, kaempferol, and their derivatives. The total content of these compounds was found to be approximately 19.45 mg/g in the leaf and the most abundant is quercetin-rutinoside (9.08 mg/g). The total phenolic content as measured by Folin-Ciocalteu assay was 261.87 ± 7.11 mg GAE/g and the total antioxidant capacity as measured in vitro was 1.46 ± 0.01 mmol Trolox/g. The antiproliferative effect of the leaf extract was measured by MTS assay with IC50 value of 239.0 ± 44.5 μg/mL. Cell-based assays show that the leaf extract inhibits the mRNA expression of proinflammatory genes (IL-6, iNOS, and TNF-α) by using RT-qPCR, implying its anti-inflammatory effects. It was further demonstrated that the underlying therapeutic mechanism involves the suppression of NF-κB, a shared pathway between cell death and inflammation.

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Citations

Jul 16, 2020·Journal of Evidence-based Integrative Medicine·Gervason MoriasiMathew Piero Ngugi
Mar 24, 2018·Journal of Food and Drug Analysis·Cara WelchQingli Wu
Aug 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Ben OlelaGervason Moriasi
Mar 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Disha VarijakzhanSwee-Hua Erin Lim

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Methods Mentioned

BETA
ABTS
PCR
Assay
protein assay

Software Mentioned

HP ChemStation Analysis

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